Laboratory of Experimental Pathophysiology, Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), 35400-000, Ouro Preto, MG, Brazil.
Laboratory of Immunobiology of Inflammation, Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), 35400-000, Ouro Preto, MG, Brazil.
Regul Toxicol Pharmacol. 2023 Aug;142:105412. doi: 10.1016/j.yrtph.2023.105412. Epub 2023 May 27.
This study aimed to evaluate long-term exposure to conventional cigarette smoke (CC) and electronic cigarette (EC) aerosol in adult male and female C57BL/6 mice. Forty-eight C57BL/6 mice were used, male (n = 24) and female (n = 24), both were divided into three groups: control, CC and EC. The CC and EC groups were exposed to cigarette smoke or electronic cigarette aerosol, respectively, 3 times a day for 60 consecutive days. Afterwards, they were maintained for 60 days without exposure to cigarettes or electronic cigarette aerosol. Both cigarettes promoted an influx of inflammatory cells to the lung in males and females. All animals exposed to CC and EC showed an increase in lipid peroxidation and protein oxidation. There was an increase of IL-6 in males and females exposed to EC. The IL-13 levels were higher in the females exposed to EC and CC. Both sexes exposed to EC and CC presented tissue damage characterized by septal destruction and increased alveolar spaces compared to control. Our results demonstrated that exposure to CC and EC induced pulmonary emphysema in both sexes, and females seem to be more susceptible to EC.
这项研究旨在评估成年雄性和雌性 C57BL/6 小鼠长期暴露于传统香烟烟雾（CC）和电子烟气溶胶中的情况。使用了 48 只 C57BL/6 小鼠，雄性（n=24）和雌性（n=24），均分为三组：对照组、CC 组和 EC 组。CC 和 EC 组分别暴露于香烟烟雾或电子烟气溶胶中，每天 3 次，连续 60 天。之后，它们被维持 60 天而不接触香烟或电子烟气溶胶。香烟和电子烟都促进了雄性和雌性肺部炎症细胞的涌入。所有暴露于 CC 和 EC 的动物都显示脂质过氧化和蛋白质氧化增加。暴露于 EC 的雄性和雌性的 IL-6 增加。暴露于 EC 和 CC 的雌性的 IL-13 水平更高。与对照组相比，暴露于 EC 和 CC 的两种性别的组织损伤都表现为隔室破坏和肺泡空间增加。我们的结果表明，暴露于 CC 和 EC 会导致两性的肺气肿，而女性似乎更容易受到 EC 的影响。
