精原干细胞移植。

Spermatogonial Transplantation.

机构信息

Department of Obstetrics and Gynecology, McGill University and The Research Institute of the McGill University Health Centre, Montreal, QC, Canada.

出版信息

Methods Mol Biol. 2023;2656:195-210. doi: 10.1007/978-1-0716-3139-3_11.

DOI:10.1007/978-1-0716-3139-3_11

PMID:37249873

Abstract

Spermatogonial transplantation is the unequivocal method to detect spermatogonial stem cells (SSCs) based strictly on the functional definition of stem cells - the cells' regenerative capacity. This method further allows for SSC quantification. A weakness of spermatogonial transplantation is its time-consuming nature; it takes 2 months to confirm the production of terminally differentiated cells in spermatogenesis, spermatozoa, in mice, which gives the assay endpoint. Using the mouse as the model system, we here describe the basic techniques of spermatogonial transplantation and provide practical guidance to successfully carry out this technique and to interpret data generated.

摘要

精原干细胞（SSCs）的鉴定基于其严格的干细胞功能性定义，即细胞的再生能力，精原细胞移植是明确检测 SSCs 的方法。这种方法还可以对 SSCs 进行定量。精原细胞移植的一个弱点是其耗时的性质；在小鼠中，需要 2 个月的时间来确认生殖细胞发生中终末分化细胞（精子）的产生，这是该检测的终点。我们在这里以小鼠为模型系统，描述了精原细胞移植的基本技术，并提供了成功实施该技术和解释产生的数据的实用指导。