Georgiev P G, Belousov L V
Ontogenez. 1986 May-Jun;17(3):256-62.
The differentiation of the presumptive mesoderm explants from different sectors of the early gastrula marginal zone was compared with that of the identical explants placed just after explantation into a medium free of divalent cations for 30 s. The development of the treated dorsal explants differed from that of control explants by the presence of well differentiated forebrain structures and the development of the explants from more ventral zones by a decrease in the occurrence of blood cells and nondifferentiated endoderm and an increase in occurrence of epithelioid structures, which form frequently deep invaginations. The shape of the treated explants was more organized and differentiated than that of the control explants. A conclusion has been reached that the development of epithelioid and forebrain structures in the explants is stimulated after a short-term disturbance of cell contacts.
将原肠胚早期边缘区不同区域的预定中胚层外植体的分化情况，与刚接种后置于无二价阳离子培养基中30秒的相同外植体的分化情况进行了比较。经处理的背侧外植体的发育与对照外植体不同，表现为出现了分化良好的前脑结构；而来自更腹侧区域的外植体发育则表现为血细胞和未分化内胚层的出现减少，上皮样结构的出现增加，上皮样结构常形成深陷窝。经处理的外植体的形态比对照外植体更有组织且分化程度更高。得出的结论是，细胞接触的短期干扰会刺激外植体中上皮样结构和前脑结构的发育。
