Department of Endodontics, School of Dentistry, Rio de Janeiro State University (UERJ), Rio de Janeiro, Brazil.
Laboratory of Experimental Surgery, School of Medical Sciences, Rio de Janeiro State University (UERJ), Rio de Janeiro, Brazil.
Int Endod J. 2023 Sep;56(9):1108-1117. doi: 10.1111/iej.13940. Epub 2023 Jun 6.
This study aimed to evaluate the volumetric change of root canal sealers through micro-computed tomographic analysis using a novel in vivo model and to compare the results with those obtained using an ex vivo test.
Eighteen single-rooted teeth were cut to 5 mm length from the root apex. The root canals were uniformly enlarged and filled with EndoSequence BC Sealer or AH Plus Jet root canal sealers. Samples were stored at 37°C and 95% relative humidity for 24 h and then scanned with a micro-CT device. Twelve samples (n = 6 for each sealer) were implanted in the subcutaneous tissue of Wistar rats, while six samples (n = 3 for each sealer) were immersed in 20 mL of phosphate-buffered saline (PBS) at 37°C at neutral pH. After 7 and 30 days, teeth were removed from subcutaneous tissue or PBS and rescanned. Statistical analysis of volume changes was performed using Shapiro-Wilk's test and independent t-test (p < .05).
AH Plus Jet had smaller volume changes (-2.2 to +0.77%) than EndoSequence BC Sealer (-2.0 to +4.0%) (p < .05), in the two tested models. The volume of the root canal sealers decreased over time (p < .05), in vivo. AH Plus Jet results varied between the in vivo and ex vivo results (p < .05), while EndoSequence BC Sealer presented similar volume losses for both experimental models (p > .05).
EndoSequence BC Sealer lost more volume than AH Plus Jet. The experimental conditions influenced the volumetric change of AH Plus Jet but not the EndoSequence BC Sealer. The ex vivo model should be further explored as a methodological alternative to assess the volumetric changes of root canal sealers without causing harm to animals.
本研究旨在通过一种新的体内模型，使用微计算机断层扫描分析评估根管封闭剂的体积变化，并将结果与体外试验进行比较。
将 18 颗单根牙从根尖切至 5mm 长。根管均匀扩大并用 EndoSequence BC 密封剂或 AH Plus Jet 根管封闭剂填充。样本在 37°C 和 95%相对湿度下储存 24 小时，然后用微 CT 设备扫描。12 个样本（每个密封剂 6 个样本）植入 Wistar 大鼠皮下组织，而 6 个样本（每个密封剂 3 个样本）在 37°C 中性 pH 的 20ml 磷酸盐缓冲液（PBS）中浸泡。7 天和 30 天后，从皮下组织或 PBS 中取出牙齿并重新扫描。使用 Shapiro-Wilk 检验和独立 t 检验对体积变化进行统计分析（p<0.05）。
在两种测试模型中，AH Plus Jet 的体积变化较小（-2.2%至+0.77%），低于 EndoSequence BC 密封剂（-2.0%至+4.0%）（p<0.05）。根管封闭剂的体积随时间而减少（p<0.05），体内试验。AH Plus Jet 的结果在体内和体外结果之间存在差异（p<0.05），而 EndoSequence BC 密封剂在两种实验模型中表现出相似的体积损失（p>0.05）。
EndoSequence BC 密封剂的体积损失大于 AH Plus Jet。实验条件影响 AH Plus Jet 的体积变化，但不影响 EndoSequence BC 密封剂。应进一步探索体外模型作为评估根管封闭剂体积变化的替代方法，而不会对动物造成伤害。
