Nagasaki International University.
Biomed Res. 2023;44(3):97-104. doi: 10.2220/biomedres.44.97.
Upregulation of the brain-derived neurotrophic factor (BDNF) in the brain can help in the prevention and treatment of depression. BDNF is synthesized in various peripheral tissues, as well as in the brain, and can reach the brain via the blood-brain barrier. Therefore, foods that upregulate peripheral BDNF levels may aid in depression management. We previously showed the BDNF-upregulating effect of white foxtail millet (WFM) using the human renal adenocarcinoma ACHN cell line, capable of producing and secreting BDNF. However, whether other varieties of foxtail millet can also upregulate BDNF is unclear. Herein, we examined the effects of red foxtail millet (RFM) on BDNF production in vitro and in vivo. RFM methanol extracts significantly increased BDNF levels in the culture medium of ACHN cells, and the levels were higher than those with WFMtreatment. Serum BDNF concentrations in rats fed a standard diet containing 20% RFM for 5 weeks were significantly higher than those in the control. Furthermore, the butanol fraction of the RFM methanol extract significantly increased BDNF levels in the culture medium of ACHN cells and upregulated BDNF mRNA expression in ACHN cells. Our results suggest that RFM has potential as a food material with BDNF-inducing activity.
脑源性神经营养因子（BDNF）在大脑中的上调有助于预防和治疗抑郁症。BDNF 在各种外周组织以及大脑中合成，并可以通过血脑屏障到达大脑。因此，上调外周 BDNF 水平的食物可能有助于抑郁症的管理。我们之前使用能够产生和分泌 BDNF 的人肾腺癌细胞系 ACHN 证明了白黍米（WFM）的 BDNF 上调作用。然而，其他品种的黍米是否也能上调 BDNF 尚不清楚。在此，我们研究了红黍米（RFM）对体外和体内 BDNF 产生的影响。RFM 甲醇提取物显著增加了 ACHN 细胞培养物中 BDNF 的水平，并且水平高于 WFM 处理。用含有 20%RFM 的标准饮食喂养 5 周的大鼠的血清 BDNF 浓度明显高于对照组。此外，RFM 甲醇提取物的丁醇级分显著增加了 ACHN 细胞培养基中的 BDNF 水平，并上调了 ACHN 细胞中的 BDNF mRNA 表达。我们的结果表明，RFM 具有作为具有 BDNF 诱导活性的食物材料的潜力。
