Wang Y, Pang K, Jacobson A E, Lessor R, Rice K C, Hoffer B
Pharmacol Biochem Behav. 1986 May;24(5):1403-7. doi: 10.1016/0091-3057(86)90202-9.
The discovery of phencyclidine (PCP) receptors has stimulated the search for specific PCP antagonists. A direct product of this research is metaphit, an irreversible PCP ligand, which has recently been synthesized. In this study we examined the effects of metaphit on the responses of hippocampal neurons to PCP. On the basis of unfiltered action potential durations, hippocampal cells were divided into two groups, complex-spike cells and theta neurons. Local application of PCP caused inhibitions of the spontaneous firing rates of complex-spike cells. Metaphit, locally applied, antagonized approximately 50% of these responses, while the remaining responses were unaffected. In contrast, PCP caused increases in the spontaneous firing rates of theta cells and in almost all cases, these responses to PCP were attenuated by metaphit administration. These effects of metaphit were specific for PCP as the responses to locally applied norepinephrine were not altered by metaphit. The data suggest two mechanisms of action of PCP in the hippocampus. In addition, these mechanisms may be localized in part to different cell types.
苯环己哌啶（PCP）受体的发现推动了对特定PCP拮抗剂的寻找。这项研究的一个直接成果是metaphit，一种不可逆的PCP配体，它最近已被合成。在本研究中，我们检测了metaphit对海马神经元对PCP反应的影响。根据未滤波的动作电位持续时间，海马细胞被分为两组，复合棘波细胞和θ神经元。局部应用PCP会抑制复合棘波细胞的自发放电率。局部应用metaphit可拮抗约50%的这些反应，而其余反应不受影响。相比之下，PCP会导致θ细胞自发放电率增加，并且在几乎所有情况下，metaphit给药都会减弱这些对PCP的反应。metaphit的这些作用对PCP具有特异性，因为局部应用去甲肾上腺素的反应不会被metaphit改变。数据表明PCP在海马体中有两种作用机制。此外，这些机制可能部分定位于不同的细胞类型。
