Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA.
Methods Mol Biol. 2023;2675:309-315. doi: 10.1007/978-1-0716-3247-5_23. Epub 2023 Jun 1.
Changes in metabolism can alter a variety of distinct cellular parameters in a number of physiological and pathological contexts. Relatedly, the loss of integrin-mediated attachment to extracellular matrix (ECM) is now appreciated to alter metabolism in a variety of distinct fashions. As such, assays to quantify and assess metabolism during ECM detachment are critical to better understanding the cellular and molecular changes that impact the behavior and survival of ECM-detached cells. Here, we discuss assays and approaches commonly used to study metabolism during ECM detachment.
代谢变化可以在许多生理和病理情况下改变多种不同的细胞参数。相关地，现在人们已经认识到整合素介导的与细胞外基质 (ECM) 的附着丧失会以多种不同的方式改变代谢。因此，量化和评估 ECM 脱离过程中代谢的测定方法对于更好地理解影响 ECM 脱离细胞行为和存活的细胞和分子变化至关重要。在这里，我们讨论了常用于研究 ECM 脱离过程中代谢的测定方法和方法。
