Shaban Nema S, Radi Abeer M, Abdelgawad Mohamed A, Ghoneim Mohammed M, Al-Serwi Rasha Hamed, Hassan Randa M, Mohammed Eman T, Radi Rania A, Halfaya Fatma M
Department of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
Department of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Aljouf 72341, Saudi Arabia.
Pharmaceuticals (Basel). 2023 Feb 8;16(2):260. doi: 10.3390/ph16020260.
Osteoarthritis (OA) represents the highest degenerative disorder. Because cartilage erosion is a common pathological alteration in OA, targeting some key metalloproteinases such as MMP-3, ADAMTS-5 besides their inhibitor TIMP-3 by natural products, could be an effective strategy to protect against osteoarthritis. Forty female Wister rats were categorized into five equal groups. Control, osteoarthritic (OA) (monosodium iodoacetate (MIA) 2 mg/50 µL saline, single intra-articular injection), OA+ indomethacin (2 mg/kg/daily/orally), OA+ nano-naringenin (25 mg/kg/daily/orally), and OA+ (772 mg/kg/daily/orally). Treatments were initiated on the 8th day after osteoarthritis induction and continued for 28 days thereafter. Finally, blood and knee joint samples were collected from all rats for biochemical and histopathological evaluations. The current study showed that MIA induced oxidative stress, which resulted in changes in the inflammatory joint markers associated with increased right knee diameter and higher clinical scores for lameness. followed by nano-naringenin exhibited a potential anti-arthritic activity by reducing the concentrations of serum MMP-3, ADAMTS-5, and joint MDA and increasing the levels of serum TIMP-3 and joint GSH, similar to indomethacin. The histopathological results confirmed these outcomes. In conclusion, and nano-naringenin can be considered as natural therapeutic agents for osteoarthritis owing to their antioxidant and anti-inflammatory activities.
骨关节炎（OA）是最常见的退行性疾病。由于软骨侵蚀是OA常见的病理改变，利用天然产物靶向一些关键的金属蛋白酶，如MMP - 3、ADAMTS - 5及其抑制剂TIMP - 3，可能是预防骨关节炎的有效策略。40只雌性Wistar大鼠被分为五组，每组数量相等。分别为对照组、骨关节炎组（碘乙酸钠（MIA）2mg/50μL生理盐水，单次关节腔内注射）、OA + 吲哚美辛组（2mg/kg/每日/口服）、OA + 纳米柚皮素组（25mg/kg/每日/口服）以及OA + （此处原文缺失具体药物名称，无法准确翻译）组（772mg/kg/每日/口服）。在诱导骨关节炎后的第8天开始治疗，并持续28天。最后，从所有大鼠采集血液和膝关节样本进行生化和组织病理学评估。当前研究表明，MIA诱导氧化应激，导致与右膝直径增加和跛行临床评分升高相关的炎症关节标志物发生变化。纳米柚皮素组随后通过降低血清MMP - 3、ADAMTS - 5和关节MDA的浓度以及增加血清TIMP - 3和关节GSH的水平，表现出潜在的抗关节炎活性，与吲哚美辛相似。组织病理学结果证实了这些结果。总之，（此处原文缺失具体药物名称，无法准确翻译）和纳米柚皮素因其抗氧化和抗炎活性可被视为骨关节炎的天然治疗剂。
