在赞比亚卢萨卡大学教学医院就诊的患者中，血清对选定抗原的抗体与活动性胃炎有关。

Serum antibodies to selected antigens are associated with active gastritis in patients seen at the University Teaching Hospital in Lusaka, Zambia.

机构信息

Tropical Gastroenterology & Nutrition group, Department of Internal Medicine, PO Box 50398, Nationalist Road, Lusaka, Zambia.

University of Zambia School of Medicine, Department of Internal Medicine, PO Box 50110, Nationalist Road, Lusaka, Zambia.

出版信息

Malawi Med J. 2022 Mar;34(1):17-24. doi: 10.4314/mmj.v34i1.4.

DOI:10.4314/mmj.v34i1.4

PMID:37265823

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10230586/

Abstract

INTRODUCTION

Little is known about specific bacterial characteristics of () infection influencing gastric carcinogenesis in Zambia. The aim of this study was to evaluate the associations between pre-selected antibodies with gastric cancer, premalignant lesions and active gastritis.

METHODS

This was cross-sectional study with multiple comparisons of patients with gastric cancer (GC), gastric premalignant (GP) lesions and active or chronic gastritis. A fluorescent bead-based antibody multiplex serology assay was used to quantify antibodies to thirteen immunogenic antigens. Logistic regression models were used to examine the associations.

RESULTS

Included were 295 patients with: 59 GC, 27 GP lesions, 48 active and 161 chronic gastritis. Overall, 257/295 (87%) were positive. seropositivity was not associated with sex, age, body mass index, socio-economic status, HIV infection, alcohol consumption or cigarette smoking (p-values all above 0.05). When compared to the patients with chronic gastritis, the presence of catalase and cinnamyl alcohol dehydrogenase (Cad) antibodies was positively associated with GP lesions (OR 3.53; 95% CI 1.52-8.17 and OR 2.47; 95% CI 1.08-5.67 respectively). However, seropositivity to Cad antibodies was significantly lower in GC patients (OR 0.28; 95% CI 0.09-0.83). Compared to chronic, active gastritis was significantly associated with (p<0.05) sero-positivity (OR 9.46; 95% CI 1.25-71.52) and specific antibodies including cytotoxin-associated gene A, vacuolating cytotoxin A, -rich protein C, hypothetical protein HP0305 and outer membrane protein HP1564.

CONCLUSIONS

Among Zambian patients seen at a single center, antibodies to (CagA, VacA, Omp, HcpC, HP0305 and HpaA) were associated with active gastritis.

摘要

简介

关于影响赞比亚胃部癌变的 () 感染的具体细菌特征知之甚少。本研究的目的是评估与胃癌、癌前病变和活动性胃炎相关的预先选定的抗体。

方法

这是一项具有多项比较的病例对照研究，包括胃癌（GC）、胃前病变（GP）和活动性或慢性胃炎患者。采用荧光珠基于抗体的多重血清学检测方法来定量 13 种免疫原性抗原的抗体。使用逻辑回归模型来检查关联。

结果

共纳入 295 例患者：59 例 GC、27 例 GP 病变、48 例活动性胃炎和 161 例慢性胃炎。总体而言，257/295（87%）为阳性。血清阳性与性别、年龄、体重指数、社会经济地位、HIV 感染、饮酒或吸烟无关（p 值均大于 0.05）。与慢性胃炎患者相比，存在过氧化氢酶和肉桂醇脱氢酶（Cad）抗体与 GP 病变呈正相关（OR 3.53；95%CI 1.52-8.17 和 OR 2.47；95%CI 1.08-5.67）。然而，Cad 抗体血清阳性在 GC 患者中显著降低（OR 0.28；95%CI 0.09-0.83）。与慢性胃炎相比，活动性胃炎与血清阳性（OR 9.46；95%CI 1.25-71.52）和特定抗体（包括细胞毒素相关基因 A、空泡细胞毒素 A、富含蛋白 C、假设蛋白 HP0305 和外膜蛋白 HP1564）显著相关（p<0.05）。