Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China.
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China.
Food Chem Toxicol. 2023 Aug;178:113870. doi: 10.1016/j.fct.2023.113870. Epub 2023 Jun 2.
L-theanine is a natural bioactive component in tea leaves and has anti-inflammatory effects. The study aimed to investigated the effects and underlying mechanisms of L-theanine on lipopolysaccharide (LPS)-induced intestinal tight junction damage in IPEC-J2 cells. Results showed that LPS induced tight junction damage by increasing reactive oxygen species production and lactate dehydrogenase (LDH) release and decreasing the mRNA expression of tight junction proteins related genes zonula occludens-1 (ZO-1, also known as Tjp1), Occludin and Claudin-1, while L-theanine reversed such an effect and attenuated the increase of p38 mitogen-activated protein kinase (p38 MAPK) mRNA expression. The p38 MAPK inhibitor (SB203580) attenuated the mRNA expression of nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (Nlrp3) inflammasome and interleukin-1β (Il-1β), and increased the mRNA expression of Tjp1, Occludin and Claudin-1, which showed a similar effect with L-theanine. In addition, NLRP3 inhibitor MCC950 attenuated the Il-1β expression and LDH release, while increased the expression of tight-junction protein-related genes. In conclusion, L-theanine could protect LPS-induced intestinal tight junction damage by inhibiting the activation of p38 MAPK-mediated NLRP3 inflammasome pathway.
L-茶氨酸是茶叶中的一种天然生物活性成分，具有抗炎作用。本研究旨在探讨 L-茶氨酸对脂多糖（LPS）诱导的 IPEC-J2 细胞肠紧密连接损伤的作用及其机制。结果表明，LPS 通过增加活性氧（ROS）的产生和乳酸脱氢酶（LDH）的释放，降低紧密连接蛋白相关基因 zonula occludens-1（ZO-1，也称为 Tjp1）、Occludin 和 Claudin-1 的 mRNA 表达，从而诱导紧密连接损伤，而 L-茶氨酸则逆转了这种作用，并减弱了 p38 丝裂原活化蛋白激酶（p38 MAPK）mRNA 表达的增加。p38 MAPK 抑制剂（SB203580）减弱了核苷酸结合寡聚结构域样受体家族含pyrin 结构域 3（Nlrp3）炎性小体和白细胞介素-1β（Il-1β）的 mRNA 表达，增加了 Tjp1、Occludin 和 Claudin-1 的 mRNA 表达，其作用与 L-茶氨酸相似。此外，NLRP3 抑制剂 MCC950 减弱了 Il-1β 的表达和 LDH 的释放，同时增加了紧密连接蛋白相关基因的表达。综上所述，L-茶氨酸通过抑制 p38 MAPK 介导的 NLRP3 炎性小体通路的激活，可保护 LPS 诱导的肠紧密连接损伤。
Zotero 插件