L-茶氨酸通过抑制 p38 MAPK 信号通路减轻 HO 诱导的 IPEC-J2 细胞炎症和凋亡。
L-theanine attenuates HO-induced inflammation and apoptosis in IPEC-J2 cells via inhibiting p38 MAPK signaling pathway.
机构信息
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China.
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China.
出版信息
Food Chem Toxicol. 2024 Apr;186:114561. doi: 10.1016/j.fct.2024.114561. Epub 2024 Mar 2.
This study investigated the protective effects of L-theanine on hydrogen peroxide (HO)-induced intestinal barrier dysfunction in IPEC-J2 cells. Results showed that L-theanine reduced HO-induced IPEC-J2 cells inflammation and apoptosis, and decreased protein phosphorylation levels of p38 mitogen-activated protein kinase (p38 MAPK) and nuclear factor kappa-B (NF-κB). The p38 MAPK inhibitor (SB203580) decreased oxidative stress, the protein expression of phosphorylation of p38 MAPK and NF-κB, the HO-induced increase in mRNA expression of pro-apoptotic and pro-inflammatory related genes expression and secretion, and tight junction protein related genes expression, which was similar to the effect of L-theanine. In conclusion, L-theanine inhibited HO-induced oxidative damage and inflammatory reaction, eliminated apoptosis, and protected intestinal epithelial barrier damage by inhibiting the activation of p38 MAPK signaling pathway.
本研究探讨了 L-茶氨酸对过氧化氢（HO）诱导的 IPEC-J2 细胞肠屏障功能障碍的保护作用。结果表明，L-茶氨酸可减轻 HO 诱导的 IPEC-J2 细胞炎症和凋亡，并降低丝裂原活化蛋白激酶 p38（p38 MAPK）和核因子 kappa-B（NF-κB）的蛋白磷酸化水平。p38 MAPK 抑制剂（SB203580）可降低氧化应激、p38 MAPK 和 NF-κB 的磷酸化蛋白表达、HO 诱导的促凋亡和促炎相关基因表达和分泌增加以及紧密连接蛋白相关基因表达，其作用与 L-茶氨酸相似。总之，L-茶氨酸通过抑制 p38 MAPK 信号通路的激活，抑制 HO 诱导的氧化损伤和炎症反应，消除细胞凋亡，从而保护肠道上皮屏障损伤。
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