通过高精度声速测量检测T4B和T7噬菌体中温度诱导的结构变化。

The detection of temperature induced structural changes in T4B and T7 bacteriophages by means of high precision acoustic velocity measurements.

作者信息

Shilnikov G V, Khusainov A A, Sarvazyan A P, Williams A R

出版信息

Ultrasound Med Biol. 1986 Jun;12(6):511-7. doi: 10.1016/0301-5629(86)90222-x.

Abstract

High precision measurements of the velocity of 7-7.5 MHz ultrasonic waves in suspensions of both T4B and T7 bacteriophages as a function of temperature revealed the presence of a distinct transition in the physiological range of 35-45 degrees C. Data from acoustic measurements, sedimentation analysis and electron microscopy enabled us to identify this transition as being caused by the protein component of the phage and not the DNA. This transition does not depend on the position of the long tail fibers and may be part of some normal physiological process within the bacteriophage which presumably enhances its recognition and attachment to its host cell.

摘要

对T4B和T7噬菌体悬浮液中7-7.5兆赫兹超声波速度随温度变化的高精度测量表明，在35-45摄氏度的生理范围内存在明显转变。声学测量、沉降分析和电子显微镜的数据使我们能够确定这种转变是由噬菌体的蛋白质成分而非DNA引起的。这种转变不依赖于长尾纤维的位置，可能是噬菌体内部某种正常生理过程的一部分，推测这一过程增强了噬菌体对宿主细胞的识别和附着。