微小 RNA10a-5p 和 PIK3CA 在中耳胆脂瘤中的表达及相关性研究。
Expression and Correlation Research of MicroRNA10a-5p and PIK3CA in Middle Ear Cholesteatoma.
机构信息
Department of Otorhinolaryngology, Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, Guangdong, People's Republic of China; Hearing Impairment and Vertigo Clinic of Zhuhai, Zhuhai Hospital of Integrated Traditional Chinese and Western Medicine, Zhuhai, Guangdong, People's Republic of China.
Department of Otorhinolaryngology, Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, Guangdong, People's Republic of China;Department of Gastroenterology, Zhuhai People's Hospital (Zhuhai Hospital affiliated with Jinan University), Zhuhai, Guangdong, People's Republic of China.
出版信息
J Int Adv Otol. 2023 Jun;19(3):212-216. doi: 10.5152/iao.2023.22863.
BACKGROUND
This study aimed to examine the roles of miR-10a-5p and phosphatidylinositol-4,5-bisphosphonate 3-kinase catalytic subunit α in the pathogenesis of middle ear cholesteatoma.
METHODS
We enrolled 27 patients with middle ear cholesteatoma and collected samples of intraoperative cholesteatoma and normal posterior ear skin tissues. The mRNA expression levels of miR-10a-5p and PIK3CA were detected using real-time quantitative polymerase chain reaction. PIK3CA protein expression was measured by immunohistochemistry and western blotting.
RESULTS
Middle ear cholesteatoma tissues showed significantly lower miR-10a-5p expression levels and significantly higher PIK3CA expression levels than normal posterior ear skin tissues (both P < .05). Furthermore, the miR-10a-5p and PIK3CA expression levels were significantly negatively correlated in middle ear cholesteatoma tissues (r = -0.926, P < .001).
CONCLUSION
Low miR-10a-5p expression levels in middle ear cholesteatoma tissues may inhibit the growth and proliferation of cholesteatoma, whereas high PIK3CA expression level may promote its growth and proliferation. In addition, miR-10a-5p may affect the proliferation and differentiation of cholesteatoma by negatively regulating its target gene, PIK3CA.
背景
本研究旨在探讨 miR-10a-5p 和磷脂酰肌醇-4,5-二磷酸 3-激酶催化亚基 α 在中耳胆脂瘤发病机制中的作用。
方法
我们纳入了 27 例中耳胆脂瘤患者，并采集了术中胆脂瘤和正常后耳皮肤组织样本。采用实时定量聚合酶链反应检测 miR-10a-5p 和 PIK3CA 的 mRNA 表达水平。通过免疫组织化学和 Western blot 检测 PIK3CA 蛋白表达。
结果
中耳胆脂瘤组织的 miR-10a-5p 表达水平明显低于正常后耳皮肤组织，而 PIK3CA 表达水平明显高于正常后耳皮肤组织（均 P <.05）。此外，中耳胆脂瘤组织中 miR-10a-5p 和 PIK3CA 的表达水平呈显著负相关（r = -0.926，P <.001）。
结论
中耳胆脂瘤组织中 miR-10a-5p 表达水平降低可能抑制胆脂瘤的生长和增殖，而 PIK3CA 表达水平升高可能促进其生长和增殖。此外，miR-10a-5p 可能通过负调控其靶基因 PIK3CA 影响胆脂瘤的增殖和分化。
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