MiR-508-3p promotes proliferation and inhibits apoptosis of middle ear cholesteatoma cells by targeting PTEN/PI3K/AKT pathway.

Cholesteatoma of the middle ear is a common disease in otolaryngology, which can lead to serious intracranial and extracranial complications. Recent studies showed that the dysregulation of microRNA may be involved in the formation of middle ear cholesteatoma. This study aimed to explore the regulatory effect of micro ribonucleic acid 508-3p () on proliferation and apoptosis of middle ear cholesteatoma cells and excavate its underlying regulatory mechanism. We found expression was upregulated in tissues and cells of cholesteatoma which was inversely related to the expression of Overexpression of could notably facilitate cholesteatoma cell proliferation. Luciferase reporter assay showed that bound the 3'-untranslated region of its downstream mRNA . Gain and loss of functions of were performed to identify their roles in the biological behaviors of cholesteatoma cells, including proliferation and apoptosis. Rescue assays confirmed that PTEN could reverse the effect of overexpression on cell proliferation. In a word, this study validated that the development of cholesteatoma may regulated by // PTEN/ PI3K/Akt axis.