Li Tian, Guo Dong, Xu Xiaoyan, Liu Peng, Wang Ping, Zhu Yongcun, Lin Lin, Qu Yemin, Liu Feng, Chu Yanliu, Gao Xiaozhong
Department of Gastroenterology, Weihai Municipal Hospital, Cheeloo College of Medicine, Shandong University, Weihai, Shandong 264200, P.R. China.
Department of Central Lab, Weihai Municipal Hospital, Cheeloo College of Medicine, Shandong University, Weihai, Shandong 264200, P.R. China.
Oncol Lett. 2023 May 12;26(1):278. doi: 10.3892/ol.2023.13864. eCollection 2023 Jul.
MicroRNA (miR/miRNA)-153, as a novel tumor-related miRNA, has been found to be aberrantly expressed in different types of cancer; however, to the best of our knowledge, the role of miR-153 in gastric cancer (GC) remains unclear. The present study demonstrated that miR-153 expression was markedly decreased in GC, including GC cell lines and culture medium, GC tissues, and serum samples, based on reverse transcription-quantitative PCR, and this was further confirmed by fluorescence hybridization. Transfection with miR-153 mimics inhibited proliferation and migration, and promoted apoptosis in GC cells. The serum expression levels of miR-153 were decreased in 59 patients with GC compared with those of 9 healthy controls, and more decreased in advanced GC compared with early-stage GC, suggesting that miR-153 was associated with tumor progression. Furthermore, serum miR-153 was expressed at significantly lower levels in patients with GC with larger tumor size (≥4 cm; P=0.013), poor differentiation and signet histology (P=0.013), lymph node metastasis (P=0.025) and advanced tumor stage (TNM stage III and IV; P=0.048) compared with patients with a smaller tumor size (<4 cm), well and moderate differentiation, no lymph node metastasis, and TNM stage I and II, respectively. In conclusion, the present study revealed that low miR-153 expression was associated with poor prognosis in GC and miR-153 may potentially act as a tumor biomarker and therapeutic target in GC.
微小RNA（miR/miRNA）-153作为一种新型的肿瘤相关微小RNA，已发现在不同类型的癌症中表达异常；然而，据我们所知，miR-153在胃癌（GC）中的作用仍不清楚。本研究表明，基于逆转录定量PCR，miR-153在GC中表达显著降低，包括GC细胞系和培养基、GC组织及血清样本，荧光杂交进一步证实了这一点。用miR-153模拟物转染可抑制GC细胞的增殖和迁移，并促进其凋亡。与9名健康对照者相比，59例GC患者血清中miR-153表达水平降低，与早期GC相比，晚期GC中miR-153表达水平降低更明显，提示miR-153与肿瘤进展相关。此外，与肿瘤较小（<4 cm）、高分化和中分化、无淋巴结转移以及TNM分期为I期和II期的患者相比，肿瘤较大（≥4 cm；P=0.013）、低分化和印戒组织学（P=0.013）、有淋巴结转移（P=0.025）以及肿瘤晚期（TNM分期为III期和IV期；P=0.048）的GC患者血清miR-153表达水平显著降低。总之，本研究表明低miR-153表达与GC患者预后不良相关，miR-153可能有望作为GC的肿瘤生物标志物和治疗靶点。
