Zhao Wei, Yin Chang-You, Jiang Jing, Kong Wei, Xu Hao, Zhang Hongtao
Department of Neurosurgery, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China.
Department of Emergency, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China.
Oncol Lett. 2019 Jan;17(1):1189-1195. doi: 10.3892/ol.2018.9706. Epub 2018 Nov 15.
Glioma is the most common and rapidly progressive type of malignant primary brain tumor in adults. miR-153 plays a major role in many malignancies; nevertheless, few studies have been conducted on glioma. The aim of the present study was to explore the role of miR-153 and SNAI1 on invasion in glioma. Reverse transcription-quantitative PCR was employed to measure the expression levels of miR-153 and SNAI1 mRNA. Transwell assay was utilized to calculate the capacity of invasion. Luciferase report assay was applied to detect whether SNAI1 was a target of miR-153. miR-153 was downregulated in glioma tissues and cells versus paracancerous tissues and normal immortalized gliocyte HEB cells. Transwell assay was used to measure whether a low expression of miR-153 in glioma indicated inhibition of cell invasion. We verified that SNAI1 was a target of miR-153 and had a negative association with miR-153 detected by luciferase reporter assay. Additionally, miR-153 suppressed cell invasive ability by regulating SNAI1 expression, whose partial function was reversed by SNAI1. miR-153 suppressed cell invasion of glioma by directly targeting SNAI1. Thus, miR-153/SNAI1 axis may be a novel target for cervical cancer treatment.
神经胶质瘤是成人中最常见且进展迅速的原发性恶性脑肿瘤类型。miR-153在许多恶性肿瘤中起主要作用；然而，针对神经胶质瘤的研究却很少。本研究的目的是探讨miR-153和SNAI1在神经胶质瘤侵袭中的作用。采用逆转录定量PCR检测miR-153和SNAI1 mRNA的表达水平。利用Transwell实验计算侵袭能力。应用荧光素酶报告基因检测法检测SNAI1是否为miR-153的靶标。与癌旁组织和正常永生化神经胶质细胞HEB细胞相比，神经胶质瘤组织和细胞中miR-153表达下调。采用Transwell实验检测神经胶质瘤中miR-153低表达是否表明细胞侵袭受到抑制。我们通过荧光素酶报告基因检测法证实SNAI1是miR-153的靶标，且与miR-153呈负相关。此外，miR-153通过调节SNAI1表达抑制细胞侵袭能力，而SNAI1可部分逆转其功能。miR-153通过直接靶向SNAI1抑制神经胶质瘤细胞的侵袭。因此，miR-153/SNAI1轴可能是神经胶质瘤治疗的新靶点。
