Bartko Ewa A, Elberling Jesper, Blom Lars H, Poulsen Lars K, Jensen Bettina M
Allergy Clinic Department of Dermatology and Allergy Copenhagen University Hospital at Gentofte Hellerup Denmark.
Skin Health Dis. 2022 Dec 14;3(3):e195. doi: 10.1002/ski2.195. eCollection 2023 Jun.
Chronic urticaria (CU) is a skin condition driven by mast cells and basophils. The exact responsiveness profile of these cells, especially regarding the anti-IgE treatment, Omalizumab, is not fully investigated. We sought to characterize the surface activation profile of basophils in CU during Omalizumab treatment and their responsiveness to IgE and non-IgE stimulation.
Whole blood basophils from 11 CU patients and 10 healthy controls were stimulated with either medium, anti-IgE, fMLP, C5a, or Substance P for 30 min and characterized by flow cytometry.
CU patients showed a broad range of basophil count as opposed to healthy subjects. An increased number of unstimulated CD69 ( = 0.05), but not CD63 basophils was observed in CU groups in comparison to healthy. The expression of CD203c and CD200R were comparable between all groups, whilst the FcεRI was reduced with the treatment. Both IgE and non-IgE mediated stimulations upregulated CD63, CD203c and CD200R, but not CD69 in all groups, however, no difference between the groups was observed. Among unstimulated basophils, expression of MRGPRX2 was higher in CU patients after Omalizumab treatment than in the healthy group (2.4% vs. 1.5%, = 0.01). The anti-IgE stimulation increased the number of MRGPRX2-expressing basophils in the CU group before and after omalizumab as compared to the healthy ( = 0.003; = 0.005). The fMLP and C5a stimulations showed a similar effect to the IgE-mediated stimulation. The MRGPRX2 ligand, Substance P did not activate basophils.
CU basophils show increased expression of MRGPRX2 after IgE and non-IgE stimulation.
慢性荨麻疹（CU）是一种由肥大细胞和嗜碱性粒细胞驱动的皮肤疾病。这些细胞的确切反应特征，尤其是关于抗IgE治疗药物奥马珠单抗的反应特征，尚未得到充分研究。我们试图描绘奥马珠单抗治疗期间CU患者嗜碱性粒细胞的表面激活特征及其对IgE和非IgE刺激的反应性。
用培养基、抗IgE、fMLP、C5a或P物质刺激11例CU患者和10名健康对照者的全血嗜碱性粒细胞30分钟，并用流式细胞术进行特征分析。
与健康受试者相比，CU患者的嗜碱性粒细胞计数范围较广。与健康组相比，CU组中未受刺激的CD69阳性嗜碱性粒细胞数量增加（P = 0.05），但CD63阳性嗜碱性粒细胞数量未增加。所有组之间CD203c和CD200R的表达相当，而治疗后FcεRI降低。IgE和非IgE介导的刺激均上调了所有组中的CD63、CD203c和CD200R，但未上调CD69，然而，各治疗组之间未观察到差异。在未受刺激的嗜碱性粒细胞中，奥马珠单抗治疗后CU患者中MRGPRX2的表达高于健康组（2.4%对1.5%，P = 0.01）。与健康组相比，抗IgE刺激增加了奥马珠单抗治疗前后CU组中表达MRGPRX2的嗜碱性粒细胞数量（P = 0.003；P = 0.005）。fMLP和C5a刺激显示出与IgE介导的刺激类似的效果。MRGPRX2配体P物质未激活嗜碱性粒细胞。
CU嗜碱性粒细胞在IgE和非IgE刺激后显示出MRGPRX2表达增加。
