静脉区域性肢体灌注时，10%二甲基亚砜溶液对站立镇静马匹腕关节氨基糖苷类药物阿米卡星药代动力学的影响。

Effects of a 10% dimethyl sulfoxide solution on radiocarpal joint amikacin pharmacokinetics during intravenous regional limb perfusion in standing sedated horses.

机构信息

Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Los Angeles, California, USA.

Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Los Angeles, California, USA.

出版信息

Vet Surg. 2023 Nov;52(8):1171-1179. doi: 10.1111/vsu.13973. Epub 2023 Jun 6.

DOI:10.1111/vsu.13973

PMID:37280731

Abstract

OBJECTIVE

To determine the effect of a 10% dimethyl sulfoxide (DMSO) solution on the peak concentration (C ) of amikacin in the radiocarpal joint (RCJ) during intravenous regional limb perfusion (IVRLP) compared with 0.9% NaCl.

STUDY DESIGN

Randomized crossover study.

ANIMALS

Seven healthy adult horses.

METHODS

The horses underwent IVRLP with 2 g of amikacin sulfate diluted to 60 mL using a 10% DMSO or 0.9% NaCl solution. Synovial fluid was collected from the RCJ at 5, 10, 15, 20, 25, and 30 minutes after IVRLP. The wide rubber tourniquet placed on the antebrachium was removed after the 30 min sample. Amikacin concentrations were quantified by a fluorescence polarization immunoassay. The mean C and time to peak concentration (T ) of amikacin within the RCJ were determined. A one-sided paired t-test was used to determine the differences between treatments. The significance level was p < .05.

RESULTS

The mean ± SD C in the DMSO group was 1361.8 ± 593 μg/mL and in the 0.9% NaCl group it was 860 ± 481.6 μg/mL (p = .058). Mean T using the 10% DMSO solution was 23 and 18 min using the 0.9% NaCl perfusate (p = .161). No adverse effects were associated with use of the 10% DMSO solution.

CONCLUSION

Although there were higher mean peak synovial concentrations using the 10% DMSO solution no difference in synovial amikacin C between perfusate type was detected (p = .058).

CLINICAL SIGNIFICANCE

Use of a 10% DMSO solution in conjunction with amikacin during IVRLP is a feasible technique and does not negatively affect the synovial amikacin levels achieved. Further research is warranted to determine other effects of using DMSO during IVRLP.

摘要

目的

比较 10%二甲基亚砜（DMSO）溶液与 0.9%生理盐水在静脉区域性肢体灌注（IVRLP）中对腕关节腔内阿米卡星峰浓度（C）的影响。

研究设计

随机交叉研究。

动物

7 匹健康成年马。

方法

马接受 IVRLP，使用 2g 硫酸阿米卡星稀释至 60mL，使用 10%DMSO 或 0.9%生理盐水溶液。在 IVRLP 后 5、10、15、20、25 和 30 分钟从腕关节采集滑液。在前臂放置的宽橡胶止血带在 30 分钟样本后取下。通过荧光偏振免疫测定法定量检测阿米卡星浓度。确定 RCJ 内阿米卡星的平均 C 和达峰时间（T）。使用单侧配对 t 检验确定两种处理方法之间的差异。显著性水平为 p<0.05。

结果

DMSO 组的平均 C 为 1361.8±593μg/mL，0.9%NaCl 组为 860±481.6μg/mL（p=0.058）。使用 10%DMSO 溶液的平均 T 为 23 分钟，使用 0.9%NaCl 灌注液的平均 T 为 18 分钟（p=0.161）。使用 10%DMSO 溶液未观察到与使用相关的不良反应。