Kimura Ichir, Ozawa Eijiro
Division of Cell Biology, National Center for Nervous, Mental, and Muscular Disorders, kodaira, Tokyo 187, Japan.
Dev Growth Differ. 1985;27(2):101-110. doi: 10.1111/j.1440-169X.1985.00101.x.
In the course of our attempt to clarify the growth-promoting activities of chick embryo extract (EE), its heat-stable activity was found to be due to hypoxanthine and its related substances including RNA. When added to a basal culture medium composed of Eagle's MEM, horse serum and Fe-saturated ovotransferrin hypoxanthine or adenine (10 μM) markedly promoted quail myoblast proliferation. The concentration of hypoxanthine in EE was very high (274±34μM) and increased 2-fold during incubation at 37°C, while that in horse serum was very low (<3 μM). Guanine, xanthine and pyrimidines were ineffective. The nucleosides and nucleotides of hypoxanthine and adenine were effective, but the deoxynucleosides strongly inhibited the proliferation of avian myoblasts. Further, RNA was also effective but DNA was not. Hypoxanthine and RNA also promoted rat myoblast proliferation and the deoxynucleosides did not inhibit rat myoblast proliferation. These findings suggest that a supply of raw materials for RNA synthesis is important for optimal proliferation of myoblasts.
在我们试图阐明鸡胚提取物（EE）的促生长活性的过程中，发现其热稳定活性归因于次黄嘌呤及其包括RNA在内的相关物质。当向由伊格尔氏最低限度基本培养基、马血清和铁饱和转铁蛋白组成的基础培养基中添加次黄嘌呤或腺嘌呤（10 μM）时，可显著促进鹌鹑成肌细胞增殖。EE中的次黄嘌呤浓度非常高（274±34μM），在37°C孵育期间增加了2倍，而马血清中的浓度非常低（<3 μM）。鸟嘌呤、黄嘌呤和嘧啶无效。次黄嘌呤和腺嘌呤的核苷和核苷酸有效，但脱氧核苷强烈抑制禽类成肌细胞的增殖。此外，RNA也有效，但DNA无效。次黄嘌呤和RNA也促进大鼠成肌细胞增殖，而脱氧核苷不抑制大鼠成肌细胞增殖。这些发现表明，RNA合成原料的供应对成肌细胞的最佳增殖很重要。
