Hamaguchi Miyako S, Hiramoto Yukio
Biological Laboratory, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152.
Department of Cell Biology, National Institute for Basic Biology, Okazaki 444, Japan.
Dev Growth Differ. 1986 Apr;28(2):143-156. doi: 10.1111/j.1440-169X.1986.00143.x.
The formation and migration of the sperm aster, and the migration of male and female pronuclei during fertilization were investigated in the eggs of the sand dollar, Clypeaster japonicus using the Colcemid-UV method. When an egg in Colcemid sea water was irradiated locally with UV light (about 365 nm wavelength) at a limited region containing sperm head, a sperm aster formed in this region, and migrated to the center of the UV-irradiated region during its formation. When the UV-irradiated region was displaced or its shape was changed after the formation of the sperm aster, the aster migrated to the center of the new UV-irradiated region. The direction of the migration of the sperm aster coincided with the direction of the longest astral rays. Direct contact between astral rays and the egg surface was not essential for sperm aster migration. When a region containing both the sperm centrosome and the female pronucleus was irradiated with UV light, the female pronucleus migrated toward the center of the sperm aster after they were connected by astral rays. The migration was suppressed when UV light was shaded over the region between the aster and the female pronucleus. These results suggest that the female pronucleus migrates to the sperm aster by attractive force between them.
利用秋水仙酰胺 - 紫外线法，对日本饼海胆（Clypeaster japonicus）卵受精过程中精子星体的形成与迁移以及雄原核和雌原核的迁移进行了研究。当处于秋水仙酰胺海水中的卵在含有精子头部的有限区域受到局部紫外线（约365纳米波长）照射时，精子星体在该区域形成，并在形成过程中迁移至紫外线照射区域的中心。精子星体形成后，若紫外线照射区域发生位移或形状改变，星体则迁移至新的紫外线照射区域的中心。精子星体的迁移方向与最长星射线的方向一致。星射线与卵表面的直接接触对于精子星体的迁移并非必需。当含有精子中心体和雌原核的区域受到紫外线照射时，雌原核在通过星射线连接后会朝着精子星体的中心迁移。当星体与雌原核之间的区域被紫外线遮挡时，迁移受到抑制。这些结果表明，雌原核通过它们之间的吸引力迁移至精子星体。
