Mahapatra Pravati Kumari, Mohanty-Hejmadi Priyambada
Department of Zoology, Utkal University, Vani Vihar, Bhubaneswar 751 004, Orissa, INDIA.
Dev Growth Differ. 1994 Jun;36(3):307-317. doi: 10.1111/j.1440-169X.1994.00307.x.
In this study the effects of vitamin A on tadpoles of Polypedates maculatus with an amputated tail were investigated. After amputation of half the tail at the hindlimb-bud stage, tadpoles were exposed to vitamin A (palmitate) 10 IU/ml solution for 24 hr (Group I), 48 hr (II), 72 hr (III), 96 hr (IV), 120 hr (V) or 144 hr (VI). Vitamin A was deleterious to survival of the tadpoles since 10, 30 and 30% tadpoles died from Group IV, V and VI, respectively before the emergence of forelimbs. Regeneration of the tail was abnormal in 91.7% of the tadpoles. Vitamin A induced homeotic transformation of the amputated tail to hindlimbs in 11.6% of the animals. Normal limb development was suppressed partially or completely in 48.3% tadpoles. These results indicate that in addition to traumatic effects, vitamin A can lead to homeotic transformation of the tail into hindlimbs and can also interfere with normal development of limbs.
在本研究中，研究了维生素A对断尾斑腿泛树蛙蝌蚪的影响。在蝌蚪后肢芽期将其尾巴截断一半后，将蝌蚪暴露于10 IU/ml的维生素A（棕榈酸酯）溶液中24小时（第一组）、48小时（第二组）、72小时（第三组）、96小时（第四组）、120小时（第五组）或144小时（第六组）。维生素A对蝌蚪的存活有害，因为在第四组、第五组和第六组中，分别有10%、30%和30%的蝌蚪在前肢出现之前死亡。91.7%的蝌蚪尾巴再生异常。维生素A在11.6%的动物中诱导断尾发生同源异型转化为后肢。48.3%的蝌蚪正常肢体发育受到部分或完全抑制。这些结果表明，除了创伤效应外，维生素A还可导致尾巴同源异型转化为后肢，并且还会干扰肢体的正常发育。
