Kanéda Iteruo
Biological Institute, Faculty of Science, Nagoya University, Chikusa-ku, Nagoya 464, Japan.
Dev Growth Differ. 1981;23(5):553-564. doi: 10.1111/j.1440-169X.1981.00553.x.
Analysis of the course of differentiation of combinants between the presumptive prechordal plate (PcP) and presumptive ectoderm (PE) by time-lapse filming showed that the PcP of early gastrulae has the capacity to induce mesoderm (notochord, muscle cells and migrating cells) in the PE. The mesoderm-inducing capacity of the PcP decreases sharply during gastrulation. Following invagination in the mid-gastrula, the PcP completely loses its mesoderm-inducing capacity. This change also occurred when the PcP of the earliest gastrula was aged in vitro for 18 hr. This shows that the mesoderm-inducing capacity of the PcP decreases autonomously with aging. PE transplanted into the presumptive trunk organizer region of the dorsal marginal zone of the earlist gastrula, became mesodermized within 12 hr. It is clear that this mesodermization of the transplanted PE is due to "tangential induction" from the PcP. The stepwise formation of the trunk organizer in Cynops pyrrhogaster is discussed in consideration of these results.
通过延时拍摄分析原索前板（PcP）和原外胚层（PE）之间组合体的分化过程表明，早期原肠胚的PcP有能力诱导PE中的中胚层（脊索、肌肉细胞和迁移细胞）。在原肠胚形成过程中，PcP的中胚层诱导能力急剧下降。在原肠胚中期内陷后，PcP完全失去其诱导中胚层的能力。当最早原肠胚的PcP在体外培养18小时后，这种变化也会发生。这表明PcP的中胚层诱导能力会随着老化而自主下降。移植到最早原肠胚背侧边缘区的假定躯干组织者区域的PE，在12小时内就会中胚层化。很明显，移植的PE的这种中胚层化是由于来自PcP的“切线诱导”。考虑到这些结果，讨论了日本林蛙躯干组织者的逐步形成过程。
