Corrected Structure of the 5' Flanking Region of Arylsulfatase Gene of the Sea Urchin, Hemicentrotus pulcherrimus: (5' flanking sequence/sea urchin/arylsulfatase gene/G-string).

The nucleotide sequence of the 5' flanking region of the arylsulfatase (Ars) gene of the sea urchin, Hemicentrotus pulcherrimus, is extensively corrected. The one previously reported from our laboratory (ref. 7, Develop, Growth & Differ., 34, 719-729, 1992) contains many sequencing errors and should be replaced with that presented here. Correction includes the addition of a 294 nucleotide (nt) sequence between -728 and -1,021. In the previous sequencing, this fragment was missed by overlooking one Hindlll site during subcloning. In addition to a cluster of direct repeats between -2,592 and -3,440, an inverted repeat is detected between -217 and -476. A possible role of the inverted repeat in the regulation of transcription of the Ars gene is discussed.