Wakamatsu Yuko, Oikawa Atsushi, Obika Masataka, Hirobe Tomohisa, Ozato Kenjiro
Biological Laboratory, Yoshida College, Kyoto University, Kyoto 606, Japan.
Department of Pharmacology, Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai 980, Japan.
Dev Growth Differ. 1984;26(5):503-513. doi: 10.1111/j.1440-169X.1984.00503.x.
Four cell lines including two sublines were established from hereditary melanomas in interspecific hybrids between platyfish (Xiphophorus maculatus) carrying the Sp gene and swordtails (X. helleri) and maintained in vitro for more than 34 months. Cells in each cell line grew randomly across each other with an apparent lack of contact inhibition of growth and at a population doubling time of 50 to 72 hr. They retained the characteristics of young pigment cells in regard to ultrastructure, tyrosinase activity, the DOPA and combined DOPA-premelanin reactions. In the degree of differentiation, the cells of the three cell lines seemed comparable to early melanocytes close to melanoblasts, and those of the remaining one cell line seemed comparable to young melanocytes but were in a more differentiated state than the early melanocytes. Colony forming ability on plastic plates was at a level of 10% in the three cell lines but only 1% in the one cell line. All four cell lines failed to form colonies in soft agar. Chromosome analysis revealed that these four cell lines were heteroploid with many abnormal figures of chromosomes and double minute chromosomes. None of the cell lines showed transplantability to fish.
从携带Sp基因的剑尾鱼（Xiphophorus helleri）与新月鱼（Xiphophorus maculatus）的种间杂种中的遗传性黑色素瘤中建立了包括两个亚系在内的四个细胞系，并在体外维持了超过34个月。每个细胞系中的细胞相互随机生长，明显缺乏生长接触抑制，群体倍增时间为50至72小时。就超微结构、酪氨酸酶活性、多巴和联合多巴-前黑素反应而言，它们保留了年轻色素细胞的特征。在分化程度上，三个细胞系的细胞似乎与接近成黑素细胞的早期黑素细胞相当，而其余一个细胞系的细胞似乎与年轻黑素细胞相当，但比早期黑素细胞处于更分化的状态。在塑料平板上的集落形成能力在三个细胞系中为10%的水平，但在一个细胞系中仅为1%。所有四个细胞系在软琼脂中均未形成集落。染色体分析显示，这四个细胞系是异倍体，有许多异常的染色体形态和双微小染色体。没有一个细胞系显示出对鱼的移植性。
