Moriyama Ryoichi, Yanagisawa Kaichiro
Institute of Biological Sciences, University of Tsukuba, Ibaraki 305, Japan.
Dev Growth Differ. 1988 Apr;30(2):169-181. doi: 10.1111/j.1440-169X.1988.00169.x.
D. discoideum has two alternative developmental pathways. If cells of two complement mating-type strains, NC4 and HM1, fuse sexually, a giant cell is produced which subsequently develops into a macrocyst, the sexual structure of this organism. However, if fusion fails to occur and cells are starved, a fruiting-body is produced instead of a macrocyst. In this paper, a two-dimensional polypeptide gel electrophoresis study showed that giant cells produce specific polypeptides which may possibly be involved in macrocyst development. Out of total 497 polypeptides which appeared in a giant cell during an incubation period of 13 hr, 92 were the specific for giant cells. Four of these polypeptides were appeared within only 1 hr after the cell fusion. The other 405 were non-specific polypeptides which appeared in both giant cells and NC4 or/and HM1 cells. However, the patterns and the rates of production of each polypeptide during the incubation period were different between these cells.
盘基网柄菌有两种交替的发育途径。如果两个互补交配型菌株NC4和HM1的细胞进行有性融合，就会产生一个巨型细胞，该细胞随后发育成大囊泡，即这种生物体的有性结构。然而，如果融合未发生且细胞饥饿，就会产生子实体而不是大囊泡。在本文中，二维多肽凝胶电泳研究表明，巨型细胞会产生特定的多肽，这些多肽可能参与大囊泡的发育。在13小时的孵育期内出现在巨型细胞中的总共497种多肽中，有92种是巨型细胞特有的。其中4种多肽在细胞融合后仅1小时内就出现了。另外405种是非特异性多肽，它们出现在巨型细胞以及NC4或/和HM1细胞中。然而，在孵育期内，这些细胞中每种多肽的产生模式和速率是不同的。
