Bulet Philippe, Kishimoto Takeo, Shirai Hiroko
Laboratory of Reproductive Biology, National Institute for Basic Biology, Myodaiji, Okazaki 444, Japan.
Dev Growth Differ. 1985;27(3):243-250. doi: 10.1111/j.1440-169X.1985.00243.x.
1-Methyladenine (1-MeAde) is the endogenous maturation-inducing substance (MIS) in starfish. However, small oocytes have no competence to 1-MeAde even at the concentration of 10 M. Furthermore, when they were injected with cytoplasm of fully-grown (large) and maturing (1-MeAde-treated) oocytes, known to contain maturation-promoting factor (MPF), they did not undergo germinal vesicle breakdown (GVBD). On the other hand, germinal vesicles (GV) of the small oocytes underwent nuclear breakdown when the small oocytes were fused with the large maturing oocytes. Therefore it is concluded that the GV of the small oocytes are capable of undergoing nuclear breakdown in the presence of the sufficient MPF, but that the small oocytes can not amplify the injected MPF. Fused cells displayed particular shape changes during the course of nuclear breakdown of both the large and the small oocytes.
1-甲基腺嘌呤（1-MeAde）是海星体内的内源性成熟诱导物质（MIS）。然而，即使在10 M的浓度下，小卵母细胞对1-MeAde也没有反应能力。此外，当向小卵母细胞注射已知含有成熟促进因子（MPF）的完全成熟（大）卵母细胞和正在成熟（经1-MeAde处理）卵母细胞的细胞质时，它们并未发生生发泡破裂（GVBD）。另一方面，当小卵母细胞与大的正在成熟的卵母细胞融合时，小卵母细胞的生发泡（GV）会发生核破裂。因此得出结论，在有足够MPF存在的情况下，小卵母细胞的GV能够发生核破裂，但小卵母细胞无法放大注入的MPF。在大、小卵母细胞核破裂过程中，融合细胞呈现出特殊的形态变化。
