Tsunematsu Yasuhiko, Coulombre Alfred J
Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Noda 278, Japan.
Laboratory of Vision Research, National Eye Institute, National Institutes of Health, U.S. Department of Health, Education and Welfare. Bethesda, Maryland 20014, USA.
Dev Growth Differ. 1981;23(4):297-311. doi: 10.1111/j.1440-169X.1981.00297.x.
The formation of neural retina (NR) from retinal pigmented epithelium (RPE) of chick embryos in culture was investigated. In cultures of explants of PRE, depigmented, preretinal foci, consisting of 50 to 100 cells appeared in the pigmented central portion of the explant within three days. Then these depigmented cells increased rapidly in number and by about day 14 they formed characteristic spherical bodies, which were identified as a neural retinal-like structure (NR structure) by electron microscopic observations. Culture of explants of RPE from embryos of different stages showed that the capacity of embryonic RPE to form an NR structure decreased steadily with embryonic age from st. 24 to 27. At and after stage 27, no foci leading to the neural retinal differentiation were formed in the explants. Medium conditioned by cell cultures of chicken embryonic NR, RPE or chondrocytes had no effect on the formation of NR structures by explants of RPE.
