关于美丽海胆胚胎孵化前的细胞周期研究

CELL CYCLE STUDY UP TO THE TIME OF HATCHING IN THE EMBRYOS OF THE SEA URCHIN, HEMICENTROTUS PULCHERRIMUS.

作者信息

Dan Katsuma, Tanaka Shoji, Yamazaki Kimie, Kato Yoshihiro

机构信息

Misaki Marine Biological Station, Koajiro, Miura-shi 238-02, Japan.

Laboratory of Developmental Biology, Mitsubishi-Kasei Institute of Life Sciences, II Minamiooya, Machida-shi, Tokyo 194, Japan.

出版信息

Dev Growth Differ. 1980;22(3):589-598. doi: 10.1111/j.1440-169X.1980.00589.x.

DOI:10.1111/j.1440-169X.1980.00589.x

PMID:37282201

Abstract

Cell cycles have been analyzed in 10 divisions up to the time of hatching in the embryos of the sea urchin, Hemicentrotus pulcherrimus. In the first 5 cleavages, division synchrony is very high. On the average, T = 55.4 min, T = 0 min, T = 12 min, T =±0 min, T = 42 min. In the remaining 5 cleavages, T becomes longer: 70 min for the 7th to 246 min for the 10th cleavage. G and G become definitely recognizable and become longer along with T . T stays more or less constant. Plots of the changing lengths of the four compartments (G , S, G , M) on the Y-axis against T (X-axis) can be fitted to the following 4 regression equations; T = 0.28T - 19.7, T = 0.609T - 15.2, T = 0.104T - 4.72 and T = 0.007T + 39.6.

摘要

在海胆胚胎孵化前，对其10次细胞分裂的细胞周期进行了分析。在前5次卵裂中，分裂同步性非常高。平均而言，第1次卵裂时长T₁ = 55.4分钟，第2次卵裂时长T₂ = 0分钟，第3次卵裂时长T₃ = 12分钟，第4次卵裂时长T₄ =±0分钟，第5次卵裂时长T₅ = 42分钟。在剩下的5次卵裂中，T变长：第7次卵裂时长为70分钟，第10次卵裂时长为246分钟。G₁期和G₂期变得明显可识别，并且随着T变长。M期时长或多或少保持恒定。以Y轴表示四个时期（G₁期、S期、G₂期、M期）的时长变化，X轴表示T，这些点可以拟合到以下4个回归方程；G₁期时长T₁ = 0.28T - 19.7，S期时长T₂ = 0.609T - 15.2，G₂期时长T₃ = 0.104T - 4.72，M期时长T₄ = 0.007T + 39.6。