Yang Hong-Yu, Zhang Ying, Wu Meng-Xi, Zhu Hui, Li Hong-Mei, Huang Lu-Qi, Tian Hui, Yuan Yuan
Guangxi University of Chinese Medicine Nanning 530200, China National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2023 May;48(10):2749-2756. doi: 10.19540/j.cnki.cjcmm.20230222.301.
The present study aimed to investigate the effect of various adjuvant rice on the quality of rice-steamed Rehmanniae Radix(RSRR) with Japonica rice, millet, yellow rice, black rice, and glutinous rice as raw materials, and analyze the anti-osteoporosis effect of RSRR by the optimal adjuvant rice. On the basis of the established UPLC-MS/MS method for the determination of the content of catalpol and rehmannioside D, comprehensive weighted scoring method was employed to evaluate the effect of various auxiliary rice on the quality of RSRR with the content of catalpol and rehmannioside D, character score, and taste score as indicators to optimize adjuvant rice. The osteoporosis model was induced by ovariectomy in rats. SD rats were randomly divided into a sham operation group, a model group, a positive control group, and low-dose and high-dose groups of Rehmanniae Radix, RSRR, steamed Rehmanniae Radix, and Epimedii Folium-RSRR. After treatment for 12 weeks, body weight, bone calcium content, and bone mineral density were mea-sured. The results showed that Japonica rice was selected as the optimal adjuvant due to the highest comprehensive score of RSRR steamed by Japonica rice. Rehmanniae Radix, RSRR, steamed Rehmanniae Radix, as well as Epimedii Folium-RSRR, could improve osteoporosis by increasing bone calcium content and bone mineral density. RSRR was superior to Rehmanniae Radix in improving osteo-porosis. However, there was no significant difference between RSRR and steamed Rehmanniae Radix. This study confirmed that Japo-nica rice was the optimal adjuvant rice of RSRR and verified the anti-osteoporosis effect of RSRR, which laid a foundation for further research on the pharmacological action and mechanism of RSRR.
本研究旨在以粳米、小米、黍米、黑米和糯米为原料，探讨不同辅助米对米蒸地黄（RSRR）品质的影响，并通过最佳辅助米分析RSRR的抗骨质疏松作用。在已建立的用于测定梓醇和地黄苷D含量的超高效液相色谱-串联质谱法的基础上，采用综合加权评分法，以梓醇和地黄苷D的含量、性状评分和口感评分为指标，评价不同辅助米对RSRR品质的影响，从而优化辅助米。通过切除大鼠卵巢诱导骨质疏松模型。将SD大鼠随机分为假手术组、模型组、阳性对照组以及生地黄、RSRR、蒸地黄和淫羊藿-RSRR的低剂量和高剂量组。治疗12周后，测量体重、骨钙含量和骨密度。结果表明，由于粳米蒸制的RSRR综合评分最高，因此选择粳米作为最佳辅助米。生地黄、RSRR、蒸地黄以及淫羊藿-RSRR均可通过增加骨钙含量和骨密度来改善骨质疏松。在改善骨质疏松方面，RSRR优于生地黄。然而，RSRR与蒸地黄之间无显著差异。本研究证实粳米是RSRR的最佳辅助米，并验证了RSRR的抗骨质疏松作用，为进一步研究RSRR的药理作用及机制奠定了基础。
