Department of Periodontology and Implantology, Buddha Institute of Dental Sciences & Hospital, Patna, Bihar, India, Phone: +91 9546150797, e-mail:
Department of Dentistry, Gandhi Medical College, Bhopal, Madhya Pradesh, India.
J Contemp Dent Pract. 2022 Sep 1;23(9):895-899. doi: 10.5005/jp-journals-10024-3372.
The aim of this research was to assess the binding of fibrin clot to the teeth affected by periodontal disease following exposure to different root conditioning agents.
A total of 60 human teeth with a solitary root that were subjected to extraction following severe periodontal disease were used as study samples in this research. Two analogous grooves were prepared on the proximal radicular surface of every sample employing a diamond-tapered fissure bur using an aerator handpiece beneath abundant irrigation. Every sample was assigned to one of the following groups: • Group I: Tetracycline hydrochloride solution • Group II: Ethylenediaminetetraacetic acid (EDTA) gel • Group III: Biopure MTAD™ Subsequent to conditioning, the samples were rinsed for 3 minutes with phosphate-buffered saline (PBS) and permitted to air-dry for 20 minutes. A drop of fresh human whole blood procured from a hale and hearty volunteer was coated onto the dentin blocks in all three groups. A scanning electron microscope under 5000× magnification at 15 kV was used to examine the samples. Kruskal-Wallis test and Mann-Whitney U test were performed to procure the inter- and intragroup assessments Results: The greatest fibrin clot union was noted in the EDTA gel group at 2.86 ± 0.14 in pursuit by Biopure MTAD™ group at 2.39 ± 0.08 as well as tetracycline hydrochloride solution group at 1.82 ± 0.10. A statistically significant difference was noted between the investigational groups ( < 0.001).
This research arrived at a conclusion that the dentinal surfaces subjected to conditioning with EDTA gel group as well as coated with human whole blood resulted in appreciably superior fibrin clot bonding to dentin vs Biopure MTAD™ as well as the tetracycline hydrochloride solution group.
Connective tissue attachment subsequent to surgical procedures causing the adhesion of a fibrin clot to the radicular surface as a result of initial wound healing processes is directly related to periodontal regeneration. It depends on biocompatibility for the fibrin clot and the periodontal pathosis-affected radicular surface to stick together, which can be procured with the aid of a variety of root conditioning measures in course of periodontal treatment.
本研究旨在评估不同根面处理剂处理牙周病患牙后，纤维蛋白凝块与牙齿的结合情况。
本研究共使用 60 颗因严重牙周病而拔除的单根人牙作为研究样本。在大量冲洗下，使用带有空气喷砂机的金刚砂锥形裂沟车针在每个样本的近中根面表面制备两个类似的沟槽。每个样本分为以下几组：• 组 I：盐酸四环素溶液• 组 II：乙二胺四乙酸（EDTA）凝胶• 组 III：Biopure MTAD™处理后，用磷酸盐缓冲盐水（PBS）冲洗 3 分钟，风干 20 分钟。在所有三组的牙本质块上涂覆一滴取自健康志愿者的新鲜全血。在 5000×放大倍数和 15 kV 下使用扫描电子显微镜进行样本检查。采用 Kruskal-Wallis 检验和 Mann-Whitney U 检验进行组间和组内评估。
EDTA 凝胶组的纤维蛋白凝块结合最强，为 2.86±0.14，其次是 Biopure MTAD™组，为 2.39±0.08，盐酸四环素溶液组为 1.82±0.10。研究组之间存在统计学显著差异（<0.001）。
本研究得出结论，与 Biopure MTAD™组和盐酸四环素溶液组相比，用 EDTA 凝胶处理并用全血涂覆的牙本质表面，纤维蛋白凝块与牙本质的结合明显更好。
手术过程中形成的连接组织附着在根面表面，由于初始伤口愈合过程，纤维蛋白凝块附着在根面表面，这与牙周再生直接相关。它取决于纤维蛋白凝块和受牙周病影响的根面表面的生物相容性，这可以通过各种根面处理措施在牙周治疗过程中获得。
