Research Center for Functional Genomics, Biomedicine, and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Translational Molecular Pathology, MD Anderson Cancer Center, Texas State University, Houston, TX, USA.
FEBS Lett. 2023 Aug;597(15):1989-2005. doi: 10.1002/1873-3468.14673. Epub 2023 Jul 9.
miRNAs are a class of noncoding RNAs with gene regulation properties, and they function as key factors in cell homeostasis. The interaction of miRNAs with their target mRNAs is largely considered to rely on sequence complementarity; however, some evidence indicates that mature miRNAs can adopt diverse conformations with implications for their function. Using the oncogenic miR-181 family as a study model, we suggest that a potential relationship between the primary sequence and secondary structure of miRNAs may have an impact on the number and spectrum of targeted cellular transcripts. We further emphasize that specific alterations in miR-181 primary sequences might impose certain constraints on target gene selection compared with the wild-type sequences, leading to the targeting of new transcripts with upregulated function in cancer.
miRNAs 是一类具有基因调控性质的非编码 RNA，它们作为细胞内稳态的关键因子发挥作用。miRNAs 与其靶 mRNAs 的相互作用很大程度上被认为依赖于序列互补性；然而，一些证据表明成熟的 miRNAs 可以采用多种构象，这对它们的功能有影响。我们以致癌的 miR-181 家族作为研究模型，提出 miRNA 的初级序列和二级结构之间可能存在潜在的关系，这可能会影响靶向细胞转录本的数量和范围。我们进一步强调，与野生型序列相比，miR-181 初级序列的特定改变可能会对靶基因的选择施加某些限制，导致靶向具有上调功能的新转录本在癌症中。
