Wei Hongjian, Wang Jiajin, Wang Qi, He Wenyuan, Liao Songkai, Huang Jiahao, Hu Wentao, Tang Ming, Chen Hui
State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Laboratory for Lingnan Modern Agriculture, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China.
Front Microbiol. 2023 May 22;14:1123632. doi: 10.3389/fmicb.2023.1123632. eCollection 2023.
Melatonin is a biomolecule that affects plant development and is involved in protecting plants from environmental stress. However, the mechanisms of melatonin's impact on arbuscular mycorrhizal (AM) symbiosis and cold tolerance in plants are still unclear. In this research, AM fungi inoculation and exogenous melatonin (MT) were applied to perennial ryegrass ( L.) seedlings alone or in combination to investigate their effect on cold tolerance. The study was conducted in two parts. The initial trial examined two variables, AM inoculation, and cold stress, to investigate the involvement of the AM fungus in endogenous melatonin accumulation and the transcriptional levels of its synthesis genes in the root system of perennial ryegrass under cold stress. The subsequent trial was designed as a three-factor analysis, encompassing AM inoculation, cold stress, and melatonin application, to explore the effects of exogenous melatonin application on plant growth, AM symbiosis, antioxidant activity, and protective molecules in perennial ryegrass subjected to cold stress. The results of the study showed that compared to non-mycorrhizal (NM) plants, cold stress promoted an increase in the accumulation of melatonin in the AM-colonized counterparts. Acetylserotonin methyltransferase (ASMT) catalyzed the final enzymatic reaction in melatonin production. Melatonin accumulation was associated with the level of expression of the genes, and . Treatment with melatonin can improve the colonization of AM fungi in plants. Simultaneous utilization of AM inoculation and melatonin treatment enhanced the growth, antioxidant activity, and phenylalanine ammonia-lyase (PAL) activity, while simultaneously reducing polyphenol oxidase (PPO) activity and altering osmotic regulation in the roots. These effects are expected to aid in the mitigation of cold stress in . Overall, melatonin treatment would help to improve growth by promoting AM symbiosis, improving the accumulation of protective molecules, and triggering in antioxidant activity under cold stress.
褪黑素是一种影响植物发育的生物分子，参与保护植物免受环境胁迫。然而，褪黑素对植物丛枝菌根（AM）共生和耐寒性的影响机制仍不清楚。在本研究中，单独或联合应用AM真菌接种和外源褪黑素（MT）处理多年生黑麦草（L.）幼苗，以研究它们对耐寒性的影响。该研究分为两个部分。初步试验考察了两个变量，即AM接种和冷胁迫，以研究在冷胁迫下AM真菌对多年生黑麦草根系内源性褪黑素积累及其合成基因转录水平的影响。后续试验设计为三因素分析，包括AM接种、冷胁迫和褪黑素处理，以探讨外源褪黑素处理对遭受冷胁迫的多年生黑麦草的植物生长、AM共生、抗氧化活性和保护分子的影响。研究结果表明，与非菌根（NM）植物相比，冷胁迫促进了AM定殖植物中褪黑素积累的增加。乙酰血清素甲基转移酶（ASMT）催化褪黑素产生的最后一步酶促反应。褪黑素积累与基因和的表达水平相关。褪黑素处理可以提高AM真菌在植物中的定殖率。同时利用AM接种和褪黑素处理可增强生长、抗氧化活性和苯丙氨酸解氨酶（PAL）活性，同时降低多酚氧化酶（PPO）活性并改变根系的渗透调节。这些作用有望有助于减轻多年生黑麦草的冷胁迫。总体而言，褪黑素处理将有助于多年生黑麦草通过促进AM共生、改善保护分子的积累以及在冷胁迫下触发抗氧化活性来促进生长。
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