Li Yanmei, Mur Luis A J, Guo Qiang, Xu Xiangnan
Institute of Plant Nutrition and Environmental Resources, Beijing Academy of Agriculture and Forestry Sciences, No. 9 Shuguanghuayuan Midroad, Haidian District, Beijing 100097, China.
Department of Life Sciences, Penglais Campus, Aberystwyth University, Aberystwyth SY23 3DA, UK.
Plants (Basel). 2025 Jul 2;14(13):2026. doi: 10.3390/plants14132026.
Chilling injury can limit the productivity of tomato ( L.), especially in over-wintering greenhouse. We here explored the effect of the pre-application of chlorogenic acid (CGA) in mitigating the impact of chilling on tomato. Flowering plants subjected to either chilling (15 °C/5 °C, day/night) or pre-treatment with CGA followed by chilling for 6 days and then by a two-day control recovery period were compared to plants maintained at control conditions (25 °C/18 °C, day/night). Chilling significantly affected the expression of PSII CP43 Chlorophyll Apoprotein, NAD (P) H-Quinone Oxidoreductase Subunit 5 and ATP Synthase CF1 Beta Subunit, reduced leaf Fv/Fm and increased malondialdehyde (MDA) levels, suggesting elevated oxidative stress. These correlated with reduced shoot biomass. All these aspects were mitigated by pretreatment with CGA. Transcriptomic and metabolomic co-analysis indicated that CGA also suppressed the shikimate pathway, phenylpropanoid biosynthesis and phenylalanine accumulation but enhanced cinnamic acid and indole acetate synthesis. Hence, the pre-chilling CGA protected the tomato plant from chilling injury by maintaining light energy utilization and reprograming secondary metabolism. This study describes the mechanism through which CGA pre-treatment can be used to maintain tomato productivity under chilling conditions.
冷害会限制番茄（L.）的产量，尤其是在越冬温室中。我们在此探究了预先施用绿原酸（CGA）对减轻低温对番茄影响的作用。将处于开花期的植株分为三组，分别是：遭受低温处理（15℃/5℃，昼/夜）的植株、预先用CGA处理然后再进行6天低温处理接着有两天对照恢复期的植株，以及维持在对照条件下（25℃/18℃，昼/夜）的植株。低温显著影响了光系统II CP43叶绿素载脂蛋白、NAD（P）H-醌氧化还原酶亚基5和ATP合酶CF1β亚基的表达，降低了叶片的Fv/Fm值并提高了丙二醛（MDA）水平，表明氧化应激加剧。这些变化与地上部生物量减少相关。CGA预处理减轻了所有这些方面的影响。转录组学和代谢组学联合分析表明，CGA还抑制了莽草酸途径、苯丙烷类生物合成和苯丙氨酸积累，但增强了肉桂酸和吲哚乙酸的合成。因此，低温前施用CGA通过维持光能利用和重新编程次生代谢，保护番茄植株免受冷害。本研究描述了CGA预处理可用于在低温条件下维持番茄产量的机制。
