Department of Biotechnology, BMS Block-1, Sector-25, Panjab University, Chandigarh, 160014, India.
Current Address: Fellow Scientist, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
Future Microbiol. 2023 Jun;18:563-580. doi: 10.2217/fmb-2022-0238. Epub 2023 Jun 7.
To decipher the role of MSMEG_5850 in the physiology of mycobacteria. was knocked out and RNA sequencing was performed. MSMEG_5850 protein was purified from the pET28a system. Electrophoretic mobility shift assay and size exclusion chromatography were used to determine the binding of MSMEG_5850 to its motif and binding stoichiometry. The effect of nutritional stress was monitored. Transcriptome analysis revealed the differential expression of 148 genes in an knockout strain. MSMEG_5850 had control over 50 genes because those genes had a binding motif upstream of their sequence. The electrophoretic mobility shift assay showed MSMEG_5850 bound to its motif as a monomer. was upregulated under nutritional stress and promoted the survival of mycobacteria. The study confirms the role of MSMEG_5850 in global transcriptional regulation.
为了解析 MSMEG_5850 在分枝杆菌生理学中的作用，对其进行了敲除，并进行了 RNA 测序。从 pET28a 系统中纯化了 MSMEG_5850 蛋白。电泳迁移率变动分析和尺寸排阻色谱法用于确定 MSMEG_5850 与其基序的结合以及结合化学计量。监测了营养应激的影响。转录组分析显示在一个 敲除菌株中差异表达了 148 个基因。MSMEG_5850 控制着 50 个基因，因为这些基因在其序列的上游有一个结合基序。电泳迁移率变动分析显示 MSMEG_5850 作为单体与其基序结合。 在营养胁迫下上调，并促进分枝杆菌的存活。 该研究证实了 MSMEG_5850 在全局转录调控中的作用。
