Dipartimento di Scienze Medico-Veterinarie, Università di Parma, Via del Taglio 10, 43126 Parma, Italy.
Dipartimento di Scienze Medico-Veterinarie, Università di Parma, Via del Taglio 10, 43126 Parma, Italy.
Environ Toxicol Pharmacol. 2023 Aug;101:104169. doi: 10.1016/j.etap.2023.104169. Epub 2023 Jun 5.
PFOA is mainly employed in products with water and oil repellent properties. Due to its persistence, bioaccumulation and critical effects on health, its use has been restricted in several countries. This research was intended to explore PFOA action on the main functions of swine ovarian granulosa cells, a valuable model for translational medicine. Moreover, since we previously demonstrated a disruptive effect on free radical generation we sought to explore PFOA effects on the main antioxidant enzymes. PFOA inhibited cell proliferation (p < 0.001), assessed by BrdU uptake. Steroidogenesis was disrupted: PFOA also stimulated 17β-estradiol production (p < 0.05), increased progesterone production (p < 0.05) at the lowest dose while it displayed an inhibitory effect at higher concentrations (p < 0.05). SOD (p < 0.001), catalase (p < 0.05) and peroxidase (p < 0.01) activities were stimulated. Therefore, our study supports a disruptive effect of PFOA in cultured swine granulosa cells.
全氟辛酸（PFOA）主要用于具有防水和防油性能的产品。由于其持久性、生物累积性和对健康的严重影响，其在多个国家的使用受到限制。本研究旨在探索 PFOA 对猪卵巢颗粒细胞主要功能的作用，猪卵巢颗粒细胞是转化医学的一个有价值的模型。此外，由于我们之前证明了其对自由基生成有破坏作用，因此我们试图探索 PFOA 对主要抗氧化酶的影响。PFOA 抑制细胞增殖（p<0.001），通过 BrdU 摄取评估。甾体生成受到干扰：PFOA 还刺激 17β-雌二醇的产生（p<0.05），在最低剂量下增加孕酮的产生（p<0.05），而在较高浓度下则显示出抑制作用（p<0.05）。SOD（p<0.001）、过氧化氢酶（p<0.05）和过氧化物酶（p<0.01）的活性受到刺激。因此，我们的研究支持 PFOA 对培养的猪卵巢颗粒细胞有破坏作用。
