Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Koodakyar St, Velenjak Ave, Chamran Highway, Tehran, 19395-4719, Iran.
Department of Biology, Faculty of Biological Sciences, Tehran North Branch, Islamic Azad University, Tehran, Iran.
Mol Biol Rep. 2023 Jul;50(7):6029-6037. doi: 10.1007/s11033-023-08533-y. Epub 2023 Jun 7.
Compared to other breast cancer subtypes, triple-negative breast cancer (TNBC) has always been challenging for clinicians due to its aggressive behavior and lack of a specific treatment. There is a confirmed association between invasive features of tumors and increased epithelial-mesenchymal transition (EMT) process, which is consistent with a higher rate of EMT in TNBC.
We investigated the expression of EMT-related genes, SNAI1 and MMP7, and EMT-related lncRNAs, treRNA and SBF2-AS1, in 50 TNBC tumors and 50 non-TNBC tumors to reveal more regulators and effectors involved in TNBC malignancy. In the present study, we showed the overexpression of all the studied genes and lncRNAs in TNBC tumors compared to non-TNBC samples. Moreover, a significant association was observed between MMP7 and treRNA expression levels and larger tumor size. A positive correlation between SNAI1 and lncRNA treRNA expression levels was also detected.
Due to the differential expression and the potential diagnostic power of the studied genes, SBF2-AS1 and treRNA can be proposed as new probable biomarkers and therapeutic targets in TNBC.
与其他乳腺癌亚型相比，三阴性乳腺癌（TNBC）因其侵袭性行为和缺乏特定治疗而一直对临床医生构成挑战。肿瘤的侵袭特征与上皮-间充质转化（EMT）过程的增加之间存在明确的关联，这与 TNBC 中 EMT 较高的发生率一致。
我们研究了 EMT 相关基因 SNAI1 和 MMP7 以及 EMT 相关 lncRNA treRNA 和 SBF2-AS1 在 50 例 TNBC 肿瘤和 50 例非 TNBC 肿瘤中的表达，以揭示更多参与 TNBC 恶性肿瘤的调节因子和效应因子。在本研究中，我们发现与非 TNBC 样本相比，所有研究基因和 lncRNA 在 TNBC 肿瘤中均过度表达。此外，还观察到 MMP7 和 treRNA 表达水平与肿瘤体积较大之间存在显著相关性。还检测到 SNAI1 和 lncRNA treRNA 表达水平之间存在正相关。
由于研究基因的差异表达及其潜在的诊断能力，SBF2-AS1 和 treRNA 可以作为 TNBC 的新的可能的生物标志物和治疗靶点。
