Institute of Experimental Neurology (INSpe), Division of Neuroscience, IRCCS San Raffaele Hospital, Milan, Italy.
Vita-Salute San Raffaele University, Milan, Italy.
BMC Neurosci. 2023 Jun 7;24(1):33. doi: 10.1186/s12868-023-00806-3.
The cross-talk between T cells and astrocytes occurring under physiological and, even more, neuroinflammatory conditions may profoundly impact the generation of adaptive immune responses in the nervous tissue. In this study, we used a standardized in vitro co-culture assay to investigate the immunomodulatory properties of astrocytes differing for age, sex, and species. Mouse neonatal astrocytes enhanced T cell vitality but suppressed T lymphocyte proliferation in response to mitogenic stimuli or myelin antigens, regardless of the Th1, Th2 or Th17 T cell phenotype. Studies comparing glia cells from adult and neonatal animals showed that adult astrocytes were more efficient in inhibiting T lymphocyte activation than neonatal astrocytes, regardless of their sex. Differently from primary cultures, mouse and human astrocytes derived from reprogrammed fibroblasts did not interfere with T cell proliferation. Overall, we describe a standardized astrocyte-T cell interaction in vitro assay and demonstrate that primary astrocytes and iAstrocytes may differ in modulating T cell function.
在生理条件下，甚至在神经炎症条件下，T 细胞和星形胶质细胞之间的串扰可能会深刻影响神经组织中适应性免疫反应的产生。在这项研究中，我们使用标准化的体外共培养测定法来研究年龄、性别和物种不同的星形胶质细胞的免疫调节特性。新生小鼠星形胶质细胞增强了 T 细胞活力，但抑制了 T 淋巴细胞对有丝分裂刺激或髓鞘抗原的增殖反应，无论 T 细胞表型是 Th1、Th2 还是 Th17。将成年和新生动物的神经胶质细胞进行比较的研究表明，成年星形胶质细胞比新生星形胶质细胞更有效地抑制 T 淋巴细胞的激活，而与它们的性别无关。与原代培养物不同的是，源自重编程成纤维细胞的小鼠和人星形胶质细胞不会干扰 T 细胞的增殖。总的来说，我们描述了一种标准化的星形胶质细胞-T 细胞体外相互作用的测定法，并证明原代星形胶质细胞和 iAstrocytes 在调节 T 细胞功能方面可能存在差异。
