qRT-PCR 验证 CENPF 上调与肺腺癌的免疫抑制状态和不良临床结局相关。

CENPF Upregulation is Associated with Immunosuppressive Status and Poor Clinical Outcomes in Lung Adenocarcinoma Validated by qRT-PCR.

机构信息

Department of Cardiothoracic Surgery, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huaian 223300, China.

出版信息

Comb Chem High Throughput Screen. 2024;27(1):78-89. doi: 10.2174/1386207326666230607125353.

DOI:10.2174/1386207326666230607125353

PMID:37287300

Abstract

OBJECTIVE

CENPF-differentially expressed in various types of cancers-is a marker of poor prognosis. However, studies on the impact of CENPF on patient prognosis in lung adenocarcinoma regarding immune infiltration are lacking.

METHODS

CENPF expression profiles were analyzed in the GEO and TCGA databases. qRT-PCR was used to verify CENPF mRNA expression in lung adenocarcinoma cell lines. The prognostic value of CENPF was evaluated by combining data from clinical samples in the GEPIA2 and TCGA databases. Metascape and WebGestalt were used for enrichment analysis of gene sets most positively associated with CENPF. Immune cell infiltration score data were retrieved from TCGA and the correlation between CENPF expression and immune cell infiltration was analyzed.

RESULTS

CENPF expression was elevated in 29 types of cancer. CENPF was highly expressed and increased with tumor grade in lung adenocarcinoma. Immunohistochemical and qRT-PCR analyses revealed that CENPF expression was upregulated in lung adenocarcinoma tissues and cells. High expression of CENPF significantly worsened prognoses in patients with multiple malignancies, including lung adenocarcinoma. Results from gene set enrichment analysis indicated significant enrichment of the progesterone-mediated oocyte maturation pathway. Immune infiltration analysis revealed that CD4 Th2 cell infiltration was significantly higher in the high CENPF expression group.

CONCLUSION

Upregulation of CENPF expression was related to poor progression-free survival, disease- free survival, and overall survival in patients with lung adenocarcinoma. High expression of CENPF was markedly related to genes associated with the immune checkpoint. Lung adenocarcinoma samples with high CENPF expression had increased CD4 Th2 cell infiltration. Our findings indicate that CENPF promotes CD4 Th2 cell infiltration through oncogenic activity and may be used as a biomarker for predicting patient outcomes in lung adenocarcinoma.

摘要

目的

CENPF 在多种癌症中差异表达，是预后不良的标志物。然而，关于 CENPF 对肺腺癌患者预后的影响与免疫浸润的研究尚缺乏。

方法

在 GEO 和 TCGA 数据库中分析 CENPF 表达谱。使用 qRT-PCR 验证肺腺癌细胞系中 CENPF mRNA 的表达。结合 GEPIA2 和 TCGA 数据库中临床样本的数据评估 CENPF 的预后价值。使用 Metascape 和 WebGestalt 对与 CENPF 最显著相关的基因集进行富集分析。从 TCGA 中检索免疫细胞浸润评分数据，并分析 CENPF 表达与免疫细胞浸润的相关性。

结果

CENPF 在 29 种癌症中表达上调。在肺腺癌中，CENPF 表达升高且随肿瘤分级而增加。免疫组化和 qRT-PCR 分析显示，CENPF 在肺腺癌组织和细胞中表达上调。CENPF 高表达显著恶化了包括肺腺癌在内的多种恶性肿瘤患者的预后。基因集富集分析结果表明孕激素介导的卵母细胞成熟途径显著富集。免疫浸润分析显示，高 CENPF 表达组 CD4 Th2 细胞浸润显著增加。

结论

CENPF 表达上调与肺腺癌患者无进展生存期、无病生存期和总生存期较差相关。CENPF 高表达与免疫检查点相关基因明显相关。高 CENPF 表达的肺腺癌样本中 CD4 Th2 细胞浸润增加。我们的研究结果表明，CENPF 通过致癌活性促进 CD4 Th2 细胞浸润，可作为预测肺腺癌患者预后的标志物。