Department of Dermatology and Venerology, Peking University First Hospital, Beijing, People's Republic of China.
Research Center for Medical Mycology, Peking University, Beijing, People's Republic of China.
Emerg Microbes Infect. 2023 Dec;12(1):2219346. doi: 10.1080/22221751.2023.2219346.
Genomes of strains of the zoophilic dermatophyte from invasive (disseminated and subcutaneous) and noninvasive (tinea capitis) infections were compared. Especially the disseminated strain showed significant syntenic rearrangements, including multiple translocations and inversions, and numerous SNPs and Indels in comparison to the noninvasive strain. In transcriptome analysis, both invasive strains were enriched for GO pathways related to components of the membrane, iron binding and heme binding, which possibly enables them to invade deeper into dermis and blood vessels. At 37 °C, invasive strains showed gene expression enriched for DNA replication, mismatch repair, N-glycan biosynthesis and ribosome biogenesis. The invasive strains were slightly less susceptible to multiple antifungal agents suggesting that acquired elevated drug resistance might be involved in the refractory disease courses. Patient with disseminated infection failed to respond to a combined antifungal treatment with itraconazole, terbinafine, fluconazole and posaconazole.
对亲动物性皮肤癣菌的侵袭性（播散性和皮下）和非侵袭性（头癣）感染菌株的基因组进行了比较。与非侵袭性菌株相比，特别是播散性菌株表现出明显的同线性重排，包括多次易位和倒位，以及大量的 SNPs 和 Indels。在转录组分析中，两种侵袭性菌株都富集了与膜成分、铁结合和血红素结合相关的 GO 途径，这可能使它们能够更深地侵入真皮和血管。在 37°C 时，侵袭性菌株表现出富含 DNA 复制、错配修复、N-聚糖生物合成和核糖体生物发生的基因表达。侵袭性菌株对多种抗真菌药物的敏感性略低，这表明获得性耐药性升高可能与难治性疾病过程有关。播散性感染患者对伊曲康唑、特比萘芬、氟康唑和泊沙康唑联合抗真菌治疗无反应。
