Song Yinggai, da Silva Nickolas Menezes, Weiss Vinicius Almir, Vu Duong, Moreno Leandro F, Vicente Vania Aparecida, Li Ruoyu, de Hoog G Sybren
Department of Dermatology and Venerology, Peking University First Hospital, Beijing, China.
Research Center for Medical Mycology, Peking University, Beijing, China.
Front Microbiol. 2020 Apr 8;11:586. doi: 10.3389/fmicb.2020.00586. eCollection 2020.
The two black yeasts and that are clinically considered as the most virulent species potentially causing disseminated infections are both producing extracellular capsule-like material, are compared. In this study, 10 genomes of and strains, including both clinical and environmental isolates, were selected based on phylogenetic analysis, physiology tests and virulence tests, sequenced on the Illumina MiSeq sequencer and annotated. Comparison of genome data were performed between intraspecific and interspecific strains. We found capsule-associated genes were however not consistently present in both species by the comparative genomics. The prevalent clinical species, , has small genomes containing significantly less virulence-associated genes than , and also than saprobic relatives. Gene OG0012246 and Myb-like DNA-binding domain and SANT/Myb domain, restricted to two strains from human brain, was shared with the neurotropic species . This study indicated that different virulence profiles existed in the two capsule-producing black yeasts, and the absence of consistent virulence-associated profiles supports the hypothesis that black yeasts are opportunists rather than primary pathogens. The results also provide the key virulence genes and drive the continuing research forward pathogen-host interactions to explore the pathogenesis.
对临床上被认为是最具潜在致病性、可能导致播散性感染的两种黑色酵母菌进行了比较，它们都会产生细胞外荚膜样物质。在本研究中，基于系统发育分析、生理学测试和毒力测试，从临床和环境分离株中选择了10株 和 菌株的基因组，在Illumina MiSeq测序仪上进行测序并注释。对种内和种间菌株的基因组数据进行了比较。然而，通过比较基因组学发现，两种酵母菌中与荚膜相关的基因并非始终存在。常见的临床菌种 基因组较小，与 相比，其毒力相关基因明显较少，与腐生亲缘种相比也是如此。基因OG0012246以及Myb样DNA结合结构域和SANT/Myb结构域仅限于来自人脑的两株菌株，与嗜神经菌种 共有。本研究表明，两种产荚膜黑色酵母菌存在不同的毒力谱，缺乏一致的毒力相关谱支持了黑色酵母菌是机会致病菌而非主要病原体的假说。研究结果还提供了关键的毒力基因，并推动了病原体-宿主相互作用的持续研究以探索发病机制。
