Lu Guili, Wang Ping, Miao Shukui, Huang Jiong, Ma Wenge, Mi Xiaoyun, Xue Jing, Shayilan Kayizha, Yang Xueyun, Yan Genqiang
College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, P. R. China.
The Veterinary Research Institute, Animal Science Academy of Xinjiang (Animal Clinical Medicine Research Center, Animal Science Academy of Xinjiang), Urumqi, Xinjiang, P. R. China.
Anim Biotechnol. 2023 Dec;34(9):5011-5015. doi: 10.1080/10495398.2023.2221703. Epub 2023 Jun 8.
In this study, we recombinantly expressed the V protein of the peste des petits ruminants virus (PPRV) and evaluated its diagnostic value for PPRV infection using an indirect ELISA (i-ELISA). The optimal concentration of the coated antigen of V protein was 15 ng/well at a serum dilution of 1:400, and the optimal positive threshold value was 0.233. A cross-reactivity assay showed that the V protein-based i-ELISA was specific to PPRV with consistent reproducibility and showed a specificity of 82.6% and a sensitivity of 100% with a virus neutralization test. Using the recombinant V protein as an antigen in ELISA is useful for seroepidemiological studies of PPRV infections.
