Ebert R F
Anal Biochem. 1986 May 1;154(2):431-5. doi: 10.1016/0003-2697(86)90010-2.
A method for analysis of phenylthiocarbamyl-amino acids by HPLC is described. Solvent A contains 0.05 M sodium acetate plus triethylamine (2.75 ml/liter) adjusted to pH 6.40 with phosphoric acid. Solvent B consists of 50% solvent A, 40% acetonitrile, and 10% methanol. The stationary phase is a 3-micron Spherisorb ODS-2 column (4.6 X 100 mm). A new sample can be injected every 27 min. Variation of the triethylamine concentration from 1 to 4 ml/liter is shown to affect column selectivity in a predictable manner, and this provides a rational basis for optimizing chromatographic conditions. Resolution of certain hydrophilic amino acids (Arg, Thr, Ala, and Pro), and mobile phase stability are improved over previously described procedures.
本文描述了一种通过高效液相色谱法分析苯硫基甲酰基氨基酸的方法。溶剂A含有0.05M醋酸钠加三乙胺（2.75ml/升），用磷酸调至pH6.40。溶剂B由50%溶剂A、40%乙腈和10%甲醇组成。固定相为3微米的Spherisorb ODS - 2柱（4.6×100mm）。每27分钟可进样一个新样品。结果表明，三乙胺浓度在1至4ml/升之间变化时，会以可预测的方式影响柱选择性，这为优化色谱条件提供了合理依据。与先前描述的方法相比，某些亲水性氨基酸（精氨酸、苏氨酸、丙氨酸和脯氨酸）的分离度以及流动相稳定性均有所提高。
