气液色谱中热离子检测法对氨基单糖的研究。应用于热球蛋白IgM（作者译）
[Study of amino monosaccharides by thermoionic detection in gas-liquid chromatography. Application to a pyroglobulin IgM (author's transl)].
作者信息
Cahour A, Hartmann L
出版信息
Pathol Biol (Paris). 1978 Oct;26(7):419-25.
The monosaccharides of proteic samples liberated by methanolysis and submitted to trimethylsilylation by the trimethylsilylimidazole (TSIM) are determined by gas-liquid chromatography. The neutral sugars are detected in simple flame ionization (FID) and the amino sugars identified separatively by the use of a thermoionic detector (TID) which responds selectively to nitrogenous and phosphorated compounds. The method described has been tested on standard mixtures of monosaccharides and on a well known glycoprotein : the uromucoid. Then it has been applied to a pyroglobulin IgM (mu2, chi2).
通过甲醇解作用释放并经三甲基硅咪唑（TSIM）进行三甲基硅烷化处理的蛋白质样品中的单糖，采用气液色谱法进行测定。中性糖通过简单的火焰离子化检测（FID）进行检测，而氨基糖则通过使用对含氮和含磷化合物有选择性响应的热离子检测器（TID）进行单独鉴定。所描述的方法已在单糖标准混合物以及一种知名糖蛋白——尿类粘蛋白上进行了测试。然后将其应用于热球蛋白IgM（μ2，χ2）。
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