Benedict Kendall L, Brady Hunter W, Newsome Anthony L
Kendall L. Benedict is a Undergraduate Research Assistants, Department of Biology, Middle Tennessee State University, Murfreesboro, TN.
Hunter W. Brady is a Undergraduate Research Assistants, Department of Biology, Middle Tennessee State University, Murfreesboro, TN.
Health Secur. 2023 Jul-Aug;21(4):303-309. doi: 10.1089/hs.2022.0138. Epub 2023 Jun 8.
The pursuit of disinfecting porous materials or fomites to inactivate viral agents has special challenges. To address these challenges, a highly portable chlorine dioxide (ClO) gas generation system was used to ascertain the ability of a gaseous preparation to inactivate a viral agent, the MS2 bacteriophage, when associated with potentially porous fomites of cloth, paper towel, and wood. The MS2 bacteriophage is increasingly used as a model to identify means of inactivating infectious viral agents of significance to humans. Studies showed that MS2 bacteriophage can be applied to and subsequently recovered from potential porous fomites such as cloth, paper towel, and wood. Paired with viral plaque assays, this provided a means for assessing the ability of gaseous ClO to inactivate bacteriophage associated with the porous materials. Notable results include 100% inactivation of 6 log bacteriophage after overnight exposure to 20 parts per million(ppm) ClO. Reducing exposure time to 90 minutes and gas ppm to lower concentrations proved to remain effective in bacteriophage elimination in association with porous materials. Stepwise reduction in gas concentration from 76 ppm to 5 ppm consistently resulted in greater than 99.99% to 100% reduction of recoverable bacteriophage. This model suggests the potential of ClO gas deployment systems for use in the inactivation of viral agents associated with porous potential fomites. The ClO gas could prove especially helpful in disinfecting enclosed areas containing viral contaminated surfaces, rather than manually spraying and wiping them.
对多孔材料或污染物进行消毒以灭活病毒制剂面临特殊挑战。为应对这些挑战，使用了一种高度便携的二氧化氯（ClO）气体生成系统，以确定一种气态制剂在与布、纸巾和木材等潜在多孔污染物相关联时灭活病毒制剂MS2噬菌体的能力。MS2噬菌体越来越多地被用作一种模型，以确定对人类具有重要意义的传染性病毒制剂的灭活方法。研究表明，MS2噬菌体可以应用于布、纸巾和木材等潜在多孔污染物，并随后从这些污染物中回收。与病毒蚀斑试验相结合，这为评估气态ClO灭活与多孔材料相关的噬菌体的能力提供了一种方法。显著的结果包括，在暴露于20百万分之一（ppm）的ClO过夜后，6对数噬菌体被100%灭活。将暴露时间缩短至90分钟，并将气体ppm浓度降至更低水平，在与多孔材料相关的噬菌体消除方面仍被证明是有效的。将气体浓度从76 ppm逐步降低到5 ppm，始终导致可回收噬菌体减少超过99.99%至100%。该模型表明了ClO气体部署系统在灭活与多孔潜在污染物相关的病毒制剂方面的潜力。ClO气体在对含有病毒污染表面的封闭区域进行消毒方面可能特别有用，而不是手动喷洒和擦拭这些区域。
