Key laboratory of the Three Gorges Reservoir Region's Eco-environments, Ministry of Education, Institute of Environment and Ecology, Chongqing University, 174 Shapingba Road, Chongqing, 400045, PR China.
Key laboratory of the Three Gorges Reservoir Region's Eco-environments, Ministry of Education, Institute of Environment and Ecology, Chongqing University, 174 Shapingba Road, Chongqing, 400045, PR China.
Water Res. 2023 Aug 1;241:120167. doi: 10.1016/j.watres.2023.120167. Epub 2023 Jun 2.
Anaerobic digestion (AD) is a favorable way to convert organic pollutants, such as food waste (FW), into clean energy through microbial action. This work adopted a side-stream thermophilic anaerobic digestion (STA) strategy to improve a digestive system's efficiency and stability. Results showed that the STA strategy brought higher methane production as well as higher system stability. It quickly adapted to thermal stimulation and increased the specific methane production from 359 mL CH/g·VS to 439 mL CH/g·VS, which was also higher than 317 mL CH/g·VS from single-stage thermophilic anaerobic digestion. Further exploration of the mechanism of STA using metagenomic and metaproteomic analysis revealed enhanced activity of key enzymes. The main metabolic pathway was up-regulated, while the dominant bacteria were concentrated, and the multifunctional Methanosarcina was enriched. These results indicate that STA optimized organic metabolism patterns, comprehensively promoted methane production pathways, and formed various energy conservation mechanisms. Further, the system's limited heating avoided adverse effects from thermal stimulation, and activated enzyme activity and heat shock proteins through circulating slurries, which improved the metabolic process, showing great application potential.
厌氧消化（AD）是一种通过微生物作用将有机污染物（如食物垃圾（FW））转化为清洁能源的有利方法。本工作采用侧流高温厌氧消化（STA）策略来提高消化系统的效率和稳定性。结果表明，STA 策略带来了更高的甲烷产量和更高的系统稳定性。它能快速适应热刺激，使特定甲烷产量从 359 毫升 CH/g·VS 增加到 439 毫升 CH/g·VS，高于单级高温厌氧消化的 317 毫升 CH/g·VS。通过宏基因组和宏蛋白质组分析进一步探索 STA 的机制表明，关键酶的活性增强。主要代谢途径被上调，而优势细菌集中，多功能产甲烷菌得到富集。这些结果表明，STA 优化了有机代谢模式，全面促进了甲烷生成途径，并形成了各种能量保护机制。此外，系统的有限加热避免了热刺激的不利影响，并通过循环泥浆激活酶活性和热休克蛋白，从而改善了代谢过程，显示出巨大的应用潜力。
