NUS Environmental Research Institute, National University of Singapore, Singapore, Singapore.
Singapore Centre for Environmental Life Sciences Engineering, National University of Singapore, Singapore, Singapore.
Sci Data. 2023 Jun 8;10(1):373. doi: 10.1038/s41597-023-02267-z.
Tropical peatlands in South-East Asia are some of the most carbon-dense ecosystems in the world. Extensive repurposing of such peatlands for forestry and agriculture has resulted in substantial microbially-driven carbon emissions. However, we lack an understanding of the microorganisms and their metabolic pathways involved in carbon turnover. Here, we address this gap by reconstructing 764 sub-species-level genomes from peat microbiomes sampled from an oil palm plantation located on a peatland in Indonesia. The 764 genomes cluster into 333 microbial species (245 bacterial and 88 archaeal), of which, 47 are near-complete (completeness ≥90%, redundancy ≤5%, number of unique tRNAs ≥18) and 170 are substantially complete (completeness ≥70%, redundancy ≤10%). The capacity to respire amino acids, fatty acids, and polysaccharides was widespread in both bacterial and archaeal genomes. In contrast, the ability to sequester carbon was detected only in a few bacterial genomes. We expect our collection of reference genomes to help fill some of the existing knowledge gaps about microbial diversity and carbon metabolism in tropical peatlands.
东南亚的热带泥炭地是世界上碳密度最高的生态系统之一。为林业和农业大规模改造这些泥炭地，导致了大量微生物驱动的碳排放。然而，我们对参与碳转化的微生物及其代谢途径缺乏了解。在这里，我们通过从印度尼西亚一个泥炭地上的油棕种植园中采集的泥炭微生物组中重建了 764 个亚种水平的基因组来填补这一空白。这 764 个基因组聚类为 333 个微生物物种（245 个细菌和 88 个古菌），其中 47 个是近完整的（完整性≥90%，冗余≤5%，独特 tRNA 的数量≥18），170 个是基本完整的（完整性≥70%，冗余≤10%）。细菌和古菌基因组中广泛存在呼吸氨基酸、脂肪酸和多糖的能力。相比之下，只有少数细菌基因组具有固碳能力。我们预计我们的参考基因组集将有助于填补关于热带泥炭地微生物多样性和碳代谢的一些现有知识空白。
