用于骨组织工程的碳纳米纤维支架上的水热增强羟基磷灰石和生物活性玻璃

Hydrothermally reinforcing hydroxyaptatite and bioactive glass on carbon nanofiber scafold for bone tissue engineering.

作者信息

Abd El-Aziz Asmaa M, Serag Eman, Kenawy Marwa Y, El-Maghraby Azza, Kandil Sherif H

机构信息

Fabrication Technology Research Department, Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City), Alexandria, Egypt.

Marine Pollution Department, Environmental Division, National Institute of Oceanography and Fisheries, Alexandria, Egypt.

出版信息

Front Bioeng Biotechnol. 2023 May 24;11:1170097. doi: 10.3389/fbioe.2023.1170097. eCollection 2023.

DOI:10.3389/fbioe.2023.1170097

PMID:37292092

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10245555/

Abstract

As a bone tissue engineering scaffold, the objective of this study was to design hierarchical bioceramics based on an electrospun composite of carbon nanofibers (CNF) reinforced with hydroxyapatite (HA) and bioactive glasses (BGs) nanoparticles. The performance of the nanofiber as a scaffold for bone tissue engineering was enhanced by reinforcing it with hydroxyapatite and bioactive glass nanoparticles through a hydrothermal process. The influence of HA and BGs on the morphology and biological properties of carbon nanofibers was examined. The prepared materials were evaluated for cytotoxicity using the water-soluble tetrazolium salt assay (WST-assay) on Osteoblast-like (MG-63) cells, and oste-ocalcin (OCN), alkaline phosphatase (ALP) activity, total calcium, total protein, and tar-trate-resistant acid phosphatase (TRAcP) were measured. The WST-1, OCN, TRAcP, total calcium, total protein, and ALP activity tests demonstrated that scaffolds reinforced with HA and BGs had excellent biocompatibility (cell viability and proliferation) and were suitable for repairing damaged bone by stimulating bioactivity and biomarkers of bone cell formation.

摘要

作为一种骨组织工程支架，本研究的目的是基于由羟基磷灰石（HA）和生物活性玻璃（BGs）纳米颗粒增强的电纺碳纳米纤维（CNF）复合材料设计分级生物陶瓷。通过水热法用羟基磷灰石和生物活性玻璃纳米颗粒增强纳米纤维作为骨组织工程支架的性能。研究了HA和BGs对碳纳米纤维形态和生物学性能的影响。使用水溶性四氮唑盐法（WST法）在成骨样（MG-63）细胞上评估所制备材料的细胞毒性，并测量骨钙素（OCN）、碱性磷酸酶（ALP）活性、总钙、总蛋白和抗酒石酸酸性磷酸酶（TRAcP）。WST-1、OCN、TRAcP、总钙、总蛋白和ALP活性测试表明，用HA和BGs增强的支架具有优异的生物相容性（细胞活力和增殖），并且通过刺激骨细胞形成的生物活性和生物标志物适用于修复受损骨骼。