Nachiappan K R, Sadasivam Balakrishnan, Najmi Ahmad, K Chandrahasan
Pharmacology, Employees' State Insurance Corporation (ESIC) Medical College & Post Graduate Institute of Medical Science and Research (PGIMSR) Model Hospital, Bangalore, IND.
Pharmacology, All India Institute of Medical Sciences (AIIMS) Bhopal, Bhopal, IND.
Cureus. 2023 May 8;15(5):e38717. doi: 10.7759/cureus.38717. eCollection 2023 May.
Background Melatonin was found to have anxiolytic properties in several animal and human studies. The melatonin receptor agonist ramelteon might also have a similar anxiolytic action. Objectives The objective of the study was to evaluate the effect of ramelteon in various rat models of anxiety and to explore the possible mechanism of action. Methods The anxiolytic effect was compared between the control group, diazepam group (1 mg/kg and 0.5 mg/kg), and ramelteon group (0.25 mg/kg, 0.5 mg/kg, and 1 mg/kg) by an elevated plus maze, light-dark box, hole board apparatus, and open field test in Sprague Dawley rats. Antagonists flumazenil, picrotoxin, and Luzindole were used to explore the possible mechanism of action of ramelteon if it showed an anxiolytic property. Results Ramelteon as a standalone drug did not show an anxiolytic effect. However, a combination of ramelteon (1 mg/kg) and diazepam (0.5 mg/kg) showed an anxiolytic effect. Conclusion Further studies can evaluate the use of a fixed-dose combination of ramelteon and already-approved anxiolytic drugs to reduce the dose of the latter.
在多项动物和人体研究中发现褪黑素具有抗焦虑特性。褪黑素受体激动剂雷美替胺可能也有类似的抗焦虑作用。目的：本研究旨在评估雷美替胺在各种大鼠焦虑模型中的作用，并探讨其可能的作用机制。方法：通过高架十字迷宫、明暗箱、洞板实验和旷场实验，比较对照组、地西泮组（1mg/kg和0.5mg/kg）以及雷美替胺组（0.25mg/kg、0.5mg/kg和1mg/kg）对Sprague Dawley大鼠的抗焦虑作用。若雷美替胺显示出抗焦虑特性，则使用拮抗剂氟马西尼、印防己毒素和鲁辛朵尔来探讨其可能的作用机制。结果：雷美替胺作为单一药物未显示出抗焦虑作用。然而，雷美替胺（1mg/kg）与地西泮（0.5mg/kg）联合使用显示出抗焦虑作用。结论：进一步的研究可以评估雷美替胺与已批准的抗焦虑药物固定剂量联合使用以减少后者剂量的情况。
