Leung Wilson, Torosin Nicole, Cao Weihuan, Reed Laura K, Arrigo Cindy, Elgin Sarah C R, Ellison Christopher E
Department of Biology, Washington University in St. Louis, St. Louis, MO 63130, USA.
Department of Genetics and Human Genetics Institute of New Jersey, Rutgers University, Piscataway, NJ 08854, USA.
bioRxiv. 2023 May 24:2023.05.22.541758. doi: 10.1101/2023.05.22.541758.
Flow cytometry estimates of genome sizes among species of show a 3-fold variation, ranging from ∼127 Mb in to ∼400 Mb in . However, the assembled portion of the Muller F Element (orthologous to the fourth chromosome in ) shows a nearly 14-fold variation in size, ranging from ∼1.3 Mb to > 18 Mb. Here, we present chromosome-level long read genome assemblies for four species with expanded F Elements ranging in size from 2.3 Mb to 20.5 Mb. Each Muller Element is present as a single scaffold in each assembly. These assemblies will enable new insights into the evolutionary causes and consequences of chromosome size expansion.
流式细胞术对[具体物种名]物种间基因组大小的估计显示出3倍的差异，范围从[具体物种1]的约127兆碱基对到[具体物种2]的约400兆碱基对。然而，穆勒F元件（与[具体物种3]的第四条染色体直系同源）的组装部分在大小上显示出近14倍的差异，范围从约1.3兆碱基对到大于18兆碱基对。在此，我们展示了四个[具体物种名]物种的染色体水平长读长基因组组装，其F元件大小扩展，范围从2.3兆碱基对到20.5兆碱基对。每个穆勒元件在每个组装中都作为单个支架存在。这些组装将为染色体大小扩展的进化原因和后果带来新的见解。
