Tang Rongbing, Ren Yanrong, Zhang Yueting, Yin Mengying, Ren Xinyu, Zhu Qiangyuan, Gao Cen, Zhang Wei, Liu Guangxiu, Liu Bin
School of Stomatology, Lanzhou University, Lanzhou, 730000, China.
Mater Today Bio. 2023 May 25;20:100678. doi: 10.1016/j.mtbio.2023.100678. eCollection 2023 Jun.
Diabetic periodontitis is a major complication of diabetes, which has a deep involvement in teeth loss and more serious systematic diseases, including Alzheimer's disease, atherosclerosis and cancers. Diabetic periodontitis is difficult to treat because of recalcitrant infection and hyperglycemia-induced tissue dysfunction. Current treatments fail to completely eliminate infection due to the diffusion-reaction inhibition of biofilm, and ignore the tissue dysfunction. Here, we design a glucose-driven transformable complex, composed of calcium alginate (CaAlg) hydrogel shell and Zeolitic imidazolate framework-8 (ZIF-8) core encapsulating Glucose oxidase (GOx)/Catalase (CAT) and Minocycline (MINO), named as CaAlg@MINO/GOx/CAT/ZIF-8 (CMGCZ). The reaction product of glucose-scavenging, gluconic acid, could dissolve ZIF-8 core and transform CMGCZ from inflexible to flexible, facilitating the complex to overcome the diffusion-reaction inhibition of biofilm. Meanwhile, reduced glucose concentration could ameliorate the pyroptosis of macrophages to decrease the secretion of pro-inflammatory factors, thereby reducing inflamm-aging to alleviate periodontal dysfunction.
糖尿病性牙周炎是糖尿病的一种主要并发症，它与牙齿脱落以及包括阿尔茨海默病、动脉粥样硬化和癌症在内的更严重的全身性疾病密切相关。由于顽固性感染和高血糖诱导的组织功能障碍，糖尿病性牙周炎难以治疗。目前的治疗方法由于生物膜的扩散反应抑制而无法完全消除感染，并且忽略了组织功能障碍。在此，我们设计了一种葡萄糖驱动的可转化复合物，其由海藻酸钙（CaAlg）水凝胶外壳和包裹葡萄糖氧化酶（GOx）/过氧化氢酶（CAT）和米诺环素（MINO）的沸石咪唑酯骨架-8（ZIF-8）核心组成，命名为CaAlg@MINO/GOx/CAT/ZIF-8（CMGCZ）。清除葡萄糖的反应产物葡萄糖酸可溶解ZIF-8核心并使CMGCZ从刚性转变为柔性，促进该复合物克服生物膜的扩散反应抑制。同时，降低的葡萄糖浓度可改善巨噬细胞的焦亡以减少促炎因子的分泌，从而减轻炎症衰老以缓解牙周功能障碍。
